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A biased solid-phase radioimmunoassay for thyroxine (T4) using T4-125I presaturated antibodies

  • Howard H. Weetall
  • Gerald Odstrchel
Article
  • 17 Downloads

Abstract

An assay for thyroxine (T4) is demonstrated using specific antibodies covalently coupled to controlled pore glass (CPG). In this assay immobilized antibodies saturated with labeled T4 are employed in a preloaded unit tube configuration. These complexes are stable for long periods of time when stored in buffer. This concept results in a highly reproducible and sensitive assay for T4 that requires a single pipetting step.

Keywords

Thyroxine Aryl Amine Merthiolate Control Pore Glass Label Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Seth, J., Rutherford, F. J., andMckenzie, I. (1975) Clin. Chem. 21: 1406.Google Scholar
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    Chopra, I. (1972) J. Clin. Endocrinol. and Metabolism 34: 938.CrossRefGoogle Scholar
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    Lynn, M., andFilbert, A. M. (1974) Bonded Stationary Phases in Chromatography, Ann Arbor Science Press, Ann Arbor, Michigan, p. 1.Google Scholar
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    Hollander, C. S., andShekman, L. (1974) Methods of Hormone Radioimmunoassay, Academic Press, New York, p. 215.Google Scholar

Copyright information

© Humana Press Inc. 1977

Authors and Affiliations

  • Howard H. Weetall
    • 1
  • Gerald Odstrchel
    • 1
  1. 1.Corning Glass WorksCorning, New York

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