Journal of Solid-Phase Biochemistry

, Volume 1, Issue 3, pp 197–208 | Cite as

Electrolytic process for regenerating nadp from nadph in a polymer matrix-bound form

  • Masuo Aizawa
  • Yoshihito Ikariyama
  • Shuichi Suzuki


Regeneration of nicotinamide adenine dinucleotide phosphate (NADP) from its reduced form (NADPH) was performed in a matrix-bound form by an electrolytic method. NADP was immobilized to alginic acid. No significant loss of coenzymic function was induced by the immobilization of NADP on the matrix. Bound NADP was soluble in water. Glucose-6-phosphate dehydrogenase (G-6-PDH) was taken as a model system of coenzyme requiring enzyme. G-6-PDH immobilized on alumina particles was coupled with the soluble form of bound NADP in a fluidized bed type of reactor. The enzymatically reduced coenzyme was electrolytically oxidized in the coenzyme regenerator of NADP from NADPH, which was found to cause no harmful loss of coenzymic function.


NADPH Alginic Acid Alumina Particle Feed Solution Anode Potential 


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Copyright information

© Humana Press Inc. 1977

Authors and Affiliations

  • Masuo Aizawa
    • 1
  • Yoshihito Ikariyama
    • 1
  • Shuichi Suzuki
    • 1
  1. 1.Research Laboratory of Resources UtilizationTokyo Institute of TechnologyTokyoJapan

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