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An enzyme immunosensor for IgG

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Abstract

An enzyme immunosensor has been developed for assaying human immunoglobulin G (IgG). The sensor is composed of an oxygen sensoring system and an antibody-binding membrane. The assay procedure involves the competitive immunochemical reaction of the membrane-bound antibody with nonlabeled and catalase-labeled IgG and the electrochemical determination of membrane-bound catalase activity. The analytical result is directly displayed by the output current of the sensor. The sensor exhibited an excellent performance in monitoring specifically human IgG.

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Authors and Affiliations

  1. Research Laboratory of Resources Utilization, Tokyo Institute of Technology Ookayama, 152, Meguro-ku Tokyo, Japan

    Masuo Aizawa, Aya Morioka, Hideaki Matsuoka & Shuichi Suzuki

  2. Department of Biochemistry, School of Medicine Fujita Gakuen University Kutsukake-cho, 470-11, Toyoake Aichi, Japan

    Yoichi Nagamura, Rikio Shinohara & Isao Ishiguro

Authors
  1. Masuo Aizawa
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  2. Aya Morioka
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  3. Hideaki Matsuoka
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  4. Shuichi Suzuki
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  5. Yoichi Nagamura
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  6. Rikio Shinohara
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  7. Isao Ishiguro
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Aizawa, M., Morioka, A., Matsuoka, H. et al. An enzyme immunosensor for IgG. Journal of Solid-Phase Biochemistry 1, 319–328 (1976). https://doi.org/10.1007/BF02990970

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  • DOI: https://doi.org/10.1007/BF02990970

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