Biological Trace Element Research

, Volume 25, Issue 1, pp 57–69 | Cite as

In vitro exchangeable erythrocytic zinc

  • Jacobus P. Van Wouwe
  • Marcel Veldhuizen
  • Jeroen J. M. De Goeij
  • Cornelis J. A. Van den Hamer


Exchangeable erythrocytic zinc is measured by65Zn uptake in and release from erythrocytes under standarized and near, physiological conditions: 7.6 μM zinc and 580 μM albumin in the medium. The intracellular exchangeable erythrocytic zinc pool in healthy volunteers amounts to 5 μmol zinc/L packed cells. The half-time of the exchange is 7 h, its activation energy 84 kJ/mol. The effects of the variation in temperature and the concentrations of albumin, as well as the effects of some zinc carriers, cell transport inhibitors, and stress hormones on the65Zn uptake are measured.

Index Entries

Erythrocytic65Zn, in vitro uptake and release of exchangeable erythrocytic zinc albumin 


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  1. 1.
    B. S. W. Smith and H. Wright,Biochim. Biophys. Acta 307, 590 (1973).PubMedCrossRefGoogle Scholar
  2. 2.
    K. E. C. De Haan, C. J. De Groot, C. J. A. Van den Hamer, and H. Boxma,Clin. Chim. Acta 170, 111 (1987).PubMedCrossRefGoogle Scholar
  3. 3.
    Analytical Methods for Atomic Absorption Spectrophotometry, Perkin-Elmer, Norwalk, CT (1976).Google Scholar
  4. 4.
    R. Whittam,Transport and diffusion in red blood cells, Edward Arnold, London, 1964, pp. 83, 126.Google Scholar
  5. 5.
    P. L. McWilliams, R. P. Agarwal, and R. I. Henkin,Clin. Res. 31, 243A (1983).Google Scholar
  6. 6.
    H. Ohno, R. Doi, K. Yamamura, K. Yamashita, S. Iizuka, and H. Taniguchi,Blut 50, 113 (1985).PubMedCrossRefGoogle Scholar
  7. 7.
    W. C. Kruckeberg and G. J. Brewer,Med. Biol. 56, 5 (1978).PubMedGoogle Scholar
  8. 8.
    G.-J. van den Berg and C. J. A. van den Hamer,J. Inorg. Biochem. 24, 289 (1985).PubMedCrossRefGoogle Scholar
  9. 9.
    A. W. Wolkoff,Hepatology 7, 777 (1987).PubMedCrossRefGoogle Scholar
  10. 10.
    W. Fuchswans and H. Springer-Lederer,Recent advances in membrane and metabolic research, E. Gerlach, K. Moser, E. Deitsch, and W. Wilmanns, eds., Thieme-Verlag, Stuttgart, FRG, 1973, pp. 121, 122.Google Scholar
  11. 11.
    R. K. Berry, M. C. Bell, and P. L. Wright,J. Nutr. 88, 284 (1966).PubMedGoogle Scholar
  12. 12.
    J. K. Chesters and M. Will,Br. J. Nutr. 38, 297 (1978).CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1990

Authors and Affiliations

  • Jacobus P. Van Wouwe
    • 1
    • 2
  • Marcel Veldhuizen
    • 2
  • Jeroen J. M. De Goeij
    • 1
  • Cornelis J. A. Van den Hamer
    • 1
  1. 1.Department of Radiochemistry, Interfaculty Reactor InstituteDelft University of TechnologyDelftthe Netherlands
  2. 2.Department of PaediatricsState University at LeidenLeidenthe Netherlands

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