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Determination of nonheme iron using inductively coupled plasma-atomic emission spectrometry

  • Katsuhiko Yokoi
  • Mieko Kimura
  • Yoshinori Itokawa
Article

Abstract

A technique for the rapid and accurate estimation of nonheme iron using inductively coupled plasma-atomic emission spectrometry is described. Yttrium was used as an internal standard. An external calibration method was used. The standards were prepared in a matrix composed of 2.5N HCl in 10% (w/v) trichloroacetic acid. The supernatant and coagulum fractions of liver nonheme iron were separated by the method of Drysdale and Ramsay with minor modification (13). The data determined by this procedure was compared and found to be agreement with data determined by the method of Hallgren (12). To evaluate the iron status of rats, hemoglobin and liver nonheme iron were determined. Hemoglobin and all of the nonheme iron fractions of the rats fed an iron-deficient diet were significantly lower than those of the rats fed an iron-sufficient diet. The blood content in the liver was estimated to be 80 μL/g from the blood iron concentration, and the difference between total and nonheme iron concentration in liver.

Index Entries

Nonheme iron inductively coupled plasma-atomic emission spectrometry yttrium rat liver blood content in liver 

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Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • Katsuhiko Yokoi
    • 1
  • Mieko Kimura
    • 1
  • Yoshinori Itokawa
    • 1
  1. 1.Department of Hygiene, Faculty of MedicineKyoto UniversityKyotoJapan

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