Determination of nonheme iron using inductively coupled plasma-atomic emission spectrometry

  • Katsuhiko Yokoi
  • Mieko Kimura
  • Yoshinori Itokawa


A technique for the rapid and accurate estimation of nonheme iron using inductively coupled plasma-atomic emission spectrometry is described. Yttrium was used as an internal standard. An external calibration method was used. The standards were prepared in a matrix composed of 2.5N HCl in 10% (w/v) trichloroacetic acid. The supernatant and coagulum fractions of liver nonheme iron were separated by the method of Drysdale and Ramsay with minor modification (13). The data determined by this procedure was compared and found to be agreement with data determined by the method of Hallgren (12). To evaluate the iron status of rats, hemoglobin and liver nonheme iron were determined. Hemoglobin and all of the nonheme iron fractions of the rats fed an iron-deficient diet were significantly lower than those of the rats fed an iron-sufficient diet. The blood content in the liver was estimated to be 80 μL/g from the blood iron concentration, and the difference between total and nonheme iron concentration in liver.

Index Entries

Nonheme iron inductively coupled plasma-atomic emission spectrometry yttrium rat liver blood content in liver 


  1. 1.
    H. N. Munro and M. C. Linder,Physiol. Rev. 58, 317–396 (1978).PubMedGoogle Scholar
  2. 2.
    B. W. Gabrio, A. Shoden, and C. A. Finch,J. Biol. Chem. 204, 815–821 (1953).PubMedGoogle Scholar
  3. 3.
    D. Drabkin,Physiol. Rev. 31, 345–431 (1951).PubMedGoogle Scholar
  4. 4.
    H. G. du Défaix, R. Puente, B. Vidal, E. Pérez, and H. Vidal,Am. J. Clin. Nutr. 33, 133–136 (1980).Google Scholar
  5. 5.
    S. C. Finch and C. A. Finch,Medicine 34, 381–430 (1955).PubMedCrossRefGoogle Scholar
  6. 6.
    S. M. Ahmed, M. Kimura, and Y. Itokawa,Trace Nutrients Research (Kyoto, Japan) 4, 121–126 (1988).Google Scholar
  7. 7.
    M. Kimura and Y. Itokawa,Magnesium in Health and Disease, Y. Itokawa and J. Durlach, eds., John Libbey, London & Paris, pp. 95–102 (1989).Google Scholar
  8. 8.
    T. L. Sourkes, K. Lloyd, and H. Birnbaum,Can. J. Biochem. 46, 267–271. (1968).PubMedCrossRefGoogle Scholar
  9. 9.
    F. H. Nielsen, S. H. Givand, and D. R. Myron,Fed. Proc. 34, 923 (1975).Google Scholar
  10. 10.
    K. Yokoi, M. Kimura, and Y. Itokawa,Biol. Trace Elem. Res. 24, 223–231 (1990).PubMedCrossRefGoogle Scholar
  11. 11.
    J. W. Drysdale and H. N. Munro,Biochem. J. 95, 851–858 (1965).PubMedGoogle Scholar
  12. 12.
    B. Hallgren,Acta Soc. Med. 59, 79–208 (1954).Google Scholar
  13. 13.
    J. W. Drysdale and W. N. M. Ramsay,Biochem. J. 95, 282–288 (1965)PubMedGoogle Scholar
  14. 14.
    S. Greenfield,Analyst (London) 105, 1032–1044 (1980).CrossRefGoogle Scholar
  15. 15.
    W. J. Boyko, P. N. Keliher, and J. M. Malloy,Anal. Chem. 52, 53R-69R (1980).CrossRefGoogle Scholar
  16. 16.
    G. J. Schmidt and W. Slavin,Anal. Chem. 54, 2491–2495 (1982).CrossRefGoogle Scholar
  17. 17.
    V. Laufberger,Bull. Soc. Chim. Biol. 19, 1575–1582 (1937).Google Scholar
  18. 18.
    J. A. Millar, R. L. C. Cumming, J. A. Smith, and A. Goldberg,Biochem. J. 119 643–649 (1970).PubMedGoogle Scholar
  19. 19.
    D. A. Lipschitz, T. H. Bothwell, H. C. Seftel, A. A. Wapnick, and R. W. Charlton,Brit. J. Haematol 20, 155–163 (1971).CrossRefGoogle Scholar
  20. 20.
    C. P. Van Wyk, M. Linder-Horowitz, and H. N. Munro,J. Biol. Chem. 246, 1025–1031 (1971).PubMedGoogle Scholar
  21. 21.
    A. Vidnes and L. Holgeland,Biochem. Biophys. Acta 328, 365–372 (1973).PubMedGoogle Scholar
  22. 22.
    A. Shukla, K. N. Agarwal, and G. S. Shukla,Biol. Trace Elem. Res. 22, 141–152 (1989).PubMedCrossRefGoogle Scholar
  23. 23.
    E. H. Morgan,J. Physiol. (London) 158, 573–586 (1961).Google Scholar
  24. 24.
    J. C. Wyllie and N. Kaufman,Brit. J. Haematol. 20, 321–327 (1971).CrossRefGoogle Scholar
  25. 25.
    E. M. Scott and R. H. McCoy,Arch. Biochem. 5, 349–355 (1944).Google Scholar
  26. 26.
    A. I. Lau and M. L. Failla,J. Nutr. 114, 224–233 (1984).PubMedGoogle Scholar
  27. 27.
    A. R. Sherman, H. A. Guthrie, and I. Wolinsky,Proc. Soc. Exptl. Biol. Med. 156, 396–401 (1977).Google Scholar
  28. 28.
    M. Uehira, Y. Endo, G. Mowlah, K. Suzuki, and S. Goto,Nutr. Rep. Int. 35, 1023–1034 (1987).Google Scholar
  29. 29.
    A. Robertson, J. N. Morrison, A. M. Wood, and I. Bremner,J. Nutr. 119, 439–445 (1989).PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • Katsuhiko Yokoi
    • 1
  • Mieko Kimura
    • 1
  • Yoshinori Itokawa
    • 1
  1. 1.Department of Hygiene, Faculty of MedicineKyoto UniversityKyotoJapan

Personalised recommendations