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Cell Biophysics

, Volume 15, Issue 3, pp 189–199 | Cite as

Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing

  • B. Bianco
  • A. Diaspro
Article

Abstract

The properties of an optical microscope are analyzed and analytically evaluated with a simple and effective model in order to understand the true meaning, limitations, and real capabilities of a defocusing technique.

Major emphasis is given to the applications related to microscopic objects of biological interest using fluorescence and absorption light microscopy. A procedure for three-dimensional viewing is analyzed and discussed.

Index Entries

Optical sectioning 3-D cell imaging Fourier optics fluorescence microscopy 3-D point spread function 

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Copyright information

© Humana Press Inc. 1989

Authors and Affiliations

  • B. Bianco
    • 1
    • 2
    • 3
  • A. Diaspro
    • 2
    • 3
  1. 1.Department of Biophysical and Electronic EngineeringGenovaItaly
  2. 2.Interdepartmental Center for Biomedical and Biophysical TechnologiesGenovaItaly
  3. 3.University of GenoaItaly

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