Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing
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The properties of an optical microscope are analyzed and analytically evaluated with a simple and effective model in order to understand the true meaning, limitations, and real capabilities of a defocusing technique.
Major emphasis is given to the applications related to microscopic objects of biological interest using fluorescence and absorption light microscopy. A procedure for three-dimensional viewing is analyzed and discussed.
Index EntriesOptical sectioning 3-D cell imaging Fourier optics fluorescence microscopy 3-D point spread function
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