Skip to main content
SpringerLink
Log in

Expression of an adhesion molecule and homing in B-cell chronic lymphocytic leukaemia: II. L-selectin expression mediated cell adhesion revealed by immobilized analogue carbohydrates in B-cell chronic lymphocytic leukaemia and monoclonal lymphocytosis of undetermined significance

  • Published:
Medical Oncology and Tumor Pharmacotherapy Aims and scope Submit manuscript

Abstract

The L-selectin mediated adhesion of freshly isolated peripheral blood mononuclear cells (PBMCs) to phosphonomonoester core polysaccharide (PPME) and fucoidin derivatized gels was investigated in seven cases of monoclonal lymphocytosis of undetermined significance (B-MLUS) and 12 cases of chronic lymphocytic leukaemia: B-CLL, patients with peripheral lymphocytosis (LY-patients), lymph node enlargement (LN-patients) and splenomegaly (SM-patients). PBMCs isolated from the peripheral blood of 10 healthy donors served as controls. The binding to PPME and fucoidin correlated well(n = 19,P = 0. 01). Adhesion of PBMCs from B-MLUS and B-CLL showed a greater variability than controls. A higher number of cells, on average, bound to PPME and fucoidin derivatized polyacrylamide gels in B-MLUS than in B-CLL. However, the differences observed were not statistically significant. In four cases with B-CLL, the stimulatory effect of interferon-α on the function of L-selectin and some other accessory molecules was also studied. The increased binding of PBMCs to immobilized analogue molecules (PPME, fucoidin) and to high endothelial venules (HEVs) in thein vitro HEV-binding assay supports the notion that interferon-α not only increases the expression of the adhesion molecules, but also results in an enhanced adhesive function.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

HEV:

high endothelial venule

B-CLL:

B-cell chronic lymphocytic leukaemia

B-MLUS:

monoclonal B lymphocytosis of undetermined significance

LN-patient:

patient with lymph node enlargement

LY-patient:

patient with peripheral lymphocytosis

SM-patient:

patient with splenomegaly

PBMC:

peripheral blood mononuclear cell

LECAM-1:

lectin cell adhesion molecule-1

LAM-1:

leucocyte adhesion molecule-1

GlyCAM:

glyosylation dependent cell adhesion molecule

LFA-1:

leucocyte functional antigen-1

ICAM-1:

intercellular adhesion molecule-1

PPME:

high-molecular-weight yeast phosphomannan core polysaccharide

PENT:

phosphorylated pentasaccharide of mannose

References

  1. Shimizu Y, Newman W, Tanaka Y, Shaw S: Lymphocyte interactions with endothelial cells.Immunol Today,13, 106 (1992).

    Article  PubMed  CAS  Google Scholar 

  2. Smith C W, Kishimoto T K, Abbas O, Hughes B, Rothlein R, Mclntire L V, Butcher E C, Anderson D C: Chemotactic factors regulate lectin adhesion molecule 1 (LECAM-1)-dependent neutrophil adhesion to cytokine-stimulated endothelial cells in vitro.J. Clin. Invest.,147, 609 (1991).

    Article  Google Scholar 

  3. Spertini O, Kansas G S, Reimann K A, Mackay C R, Tedder T F: Function and evolutionary conservation of distinct epitopes on the leukocyte adhesion molecule-1 (LAM-1) that regulate leukocyte migration.J. Immunol.,147, 942 (1991).

    PubMed  CAS  Google Scholar 

  4. Camerini D, James S P, Stamenkovic T, Seed B: Leu-8/TQl is the human equivalent of the Mel-14 lymph node homing receptor.Nature,342, 78 (1989).

    Article  PubMed  CAS  Google Scholar 

  5. Imai Y, Singer M S, Fennie C, Lasky L A, Rosen S D: Identification of a carbohydrate-based endothelial ligand for a lymphocyte homing receptor.J. Cell Biol.,113, 1213 (1991).

    Article  PubMed  CAS  Google Scholar 

  6. Lasky L A, Singer M S, Dowbenko D, Imai Y, Henzel W J, Grimley C, Fennie C, Gillett N, Watson S N, Rosen S D: An endothelial ligand for L-selectin is a novel mucin-like molecule.Cell,69, 927 (1992).

    Article  PubMed  CAS  Google Scholar 

  7. Springer T A, Lasky L A: Sticky sugars for selectins.Nature,349, 196 (1991).

    Article  PubMed  CAS  Google Scholar 

  8. Brandley B K, Ross T S, Schnaar R L: Multiple carbohydrate receptors on lymphocytes revealed by adhesion to immobilized polysaccharides.J. Cell Biol.,105, 991 (1987).

    Article  PubMed  CAS  Google Scholar 

  9. Csanaky G, Vass J A: L-selectins revealed by immobilized analogue molecules on human peripheral blood lymphocytes.Acta Biol Sci. Hung. (in press).

  10. Yednock T A, Stoolman L M, Rosen S D: Phosphomannosyl- derivatized beads detect a receptor involved in lymphocyte homing.J. Cell Biol.,104, 713 (1987).

    Article  PubMed  CAS  Google Scholar 

  11. Spertini O, Freedman A S, Belvin M P, Penta A C, Griffin J D, Tedder T F: Regulation of leukocyte adhesion molecule-1 (TQ1, Leu-8) expression and shedding by normal and malignant cells.Leukemia,5, 300 (1991).

    PubMed  CAS  Google Scholar 

  12. Jewell A P, Yong K L, Worman C P, Giles F J, Goldstone A H, Lydyard P M: Cytokine induction of leukocyte adhesion molecule-1 (LAM-1) expression on chronic lymphocytic leukaemia cells.Leukemia,6, 400 (1992).

    PubMed  CAS  Google Scholar 

  13. Picker L J, Medeiros L J, Weiss L M, Warnke R A, Butcher E C: Expression of lymphocyte homing receptor antigen in non-Hodgkin’s lymphoma.Am. J. Pathol.,130, 496 (1988).

    PubMed  CAS  Google Scholar 

  14. Pals S T, Horst E, Ossekoppele G J, Figdor C G, Scheper R J, Meijer C J L M: Expression of lymphocyte homing receptor as a mechanism of dissemination in non-Hodgkin’s lymphoma.Blood,73, 885 (1989).

    PubMed  CAS  Google Scholar 

  15. Möller P, Eichelmann A, Leithäuser F, Mechtersheimer G, Herwart F O: Venular endothelium binding molecules CD44 and LECAM-1 in normal and malignant B-cell populations. A comparative study.Virchows. Archiv. A Pathol. Anal.,421, 305 (1992).

    Article  Google Scholar 

  16. Jansen J H, van der Harst D, Wientjens G J H M, Kooy-Winkelaar Y M C, Brand A, Willemze R, Kluin-Nelemans H C: Induction of CD 11a/leukocyte function antigen-1 and CD54/intercellular adhesion molecule-1 on hairy cell leukemia cells is accompanied by enhanced susceptibility to T-cell but not lymphokine-activated killer-cell cytotoxicity.Blood,80, 478 (1992).

    PubMed  CAS  Google Scholar 

  17. Marotta G, Zagonel V, Pinto A: Induction of LFA-1/CD11a and ICAM-1/CD54 adhesion molecules on neoplastic B cells during in vivo treatment of chronic lymphocytic leukemia with interferon-α2. Blood,81, 267 (1993).

    PubMed  CAS  Google Scholar 

  18. Kansas G, Dailey M O: Expression of adhesion structures during B cell development in man.J. Immunol.,142, 3058 (1989).

    PubMed  CAS  Google Scholar 

  19. Tedder T F, Penta A C, Levine H B, Freedman A S: Expression of the human leukocyte adhesion molecule, LAM-1: identity with the TQ1 and Leu-8 differentiation antigens.J. Immunol,144, 532 (1990).

    PubMed  CAS  Google Scholar 

  20. Kimby E, Mellstedt H, Björkholm M, Holm G: Clonal cell surface structures related to differentiation, activation and homing in B-cell chronic lymphocytic leukemia and monoclonal lymphocytosis of undetermined significance.Eur. J. Haematol,43, 452 (1989).

    Article  PubMed  CAS  Google Scholar 

  21. Csanaky G, Kalász V, Kelényi G, Losonczy H, Balikó Z, Tóth A: Expression of an adhesion molecule and homing in B-cell chronic lymphocytic leukaemia I. Application of the HEV-binding assay to a clinical series.Med. Oncol. Turn. Pharmac.,10, (1993).

  22. Raja R H, Leboeuf R D, Stone G W, Weigel P: Preparation of alky lamine and 1251-radiolabeled derivatives of hyaluronic acid uniquely modified at the reducing end.Anal. Biochem.,139, 168 (1984).

    Article  PubMed  CAS  Google Scholar 

  23. Guarnaccia S P, Schnaar R L: Hepatocyte adhesion to immobilized carbohydrates I. Sugar recognition is followed by energy-dependent strengthening.J. Biol. Chem.,257, 14288 (1982).

    PubMed  CAS  Google Scholar 

  24. Pless D D, Lee C, Roseman S, Schnaar R L: Specific cell adhesion to immobilized glycoproteins demonstrated using new reagents for protein and glycoprotein immobilization.J. Biol. Chem.,258, 2340 (1983).

    PubMed  CAS  Google Scholar 

  25. Schnaar R L, Lee Y C: Polyacry lamide gels copolymerized with active esters. A new medium for affinity systems.Biochemistry,14, 1535 (1975).

    Article  PubMed  CAS  Google Scholar 

  26. Ley K, Linnemann G, Meinen M, Stoolman L M, Gaehtgens P: Fucoidin, but not yeast polyphosphomannan PPME, inhibits leukocyte rolling in venules of rat mesentery.Blood,81, 177 (1993).

    PubMed  CAS  Google Scholar 

  27. Parish C R, Hogarth P M, McKenzie I F: Evidence that Thy-1 and Ly-5 (T-200) antigens interact with sulphated carbohydrates.Immunol Cell Biol.,66, 221 (1988).

    Article  PubMed  CAS  Google Scholar 

  28. Spertini O, Kansas G S, Reiman K A, Mac Kay C R, Tedder T F: Function and evolutionary conservation of distinct epitopes on the leukocyte adhesion molecule-1 (TQ-1, Leu-8) that regulate leukocyte migration.J. Immunol.,147, 942 (1991).

    PubMed  CAS  Google Scholar 

  29. Spertini O, Kansas G S, Munro J M, Griffin J D, Tedder T F: Regulation of leukocyte migration by activation of leukocyte adhesion molecule-1 (LAM-1) selectin.Nature,349, 691 (1991).

    Article  PubMed  CAS  Google Scholar 

  30. Yednock T A, Butcher E C, Stoolman L M, Rosen S D: Receptors involved in lymphocyte homing: Relationship between a carbohydrate-binding receptor and the Mel-14 antigen.J. Cell Biol.,104, 725 (1987).

    Article  PubMed  CAS  Google Scholar 

  31. Watson S, Imai Y, Fennie C, Geoffroy J S, Rosen S D, Lasky L A: A homing receptor-IgG chimera as a probe for adhesive Iigands of lymph node high endothelial venules.J. Cell Biol.,110, 2221 (1990).

    Article  PubMed  CAS  Google Scholar 

  32. Salmi M, Jalkanen S: Regulation of L-selectin expression on cultured bone marrow leukocytes and their precursors.Eur. J. Immunol.,22, 835 (1992).

    Article  Google Scholar 

  33. Ohgama J, Onoé K: Quantitative analysis of Mel-14 expression on various lymphocyte subpopulations.Immunobiol.,186, 268 (1992).

    CAS  Google Scholar 

  34. Hamann A, Jablonski-Westrich D, Scholz K U, Duijvestijn A, Butcher E C, Thiele H G: Regulation of lymphocyte homing. I. Alterations in homing receptor expression and organ-specific high endothelial venule binding of lymphocytes upon activation.J. Immunol.,140, 737 (1988).

    PubMed  CAS  Google Scholar 

  35. Buhrer C, Berlin C, Thiele H G, Hamann A: Lymphocyte activation and expression of the human leukocyte-endothelial cell adhesion molecule-1 (Leu-8/TQ1 antigen).Immunology,71, 442 (1990).

    PubMed  CAS  Google Scholar 

  36. Buhrer C, Berlin C, Jablonski-Westrich D, Holzmann B, Thiele H G, Hamann A: Lymphocyte activation and regulation of three adhesion molecules with supposed function in homing: LECAM-1 (MEL-14 antigen), LPAM-1/2 (alfa 4-integrin) and CD44.Scand. J. Immunol.,35, 107 (1992).

    Article  PubMed  CAS  Google Scholar 

  37. Hamann A, Jablonski-Westrich D, Duijvestijn A, Butcher E C, Baisch H, Harder R, Thiele H G, Evidence for an accessory role of LFA-1 in lymphocyte-high endothelium interaction during homing.J. Immunol.,140, 693 (1988).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, University Medical School of Pécs, H-7643, Pécs, Hungary

    György Csanaky & János A. Vass

  2. 1st Department of Internal Medicine, University Medical School of Pécs, H-7643, Pécs, Hungary

    Hajna Losonczy

  3. 2nd Department of Internal Medicine, University Medical School of Pécs, H-7643, Pécs, Hungary

    Matild Schmelczer

Authors
  1. György Csanaky
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. János A. Vass
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hajna Losonczy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Matild Schmelczer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Csanaky, G., Vass, J.A., Losonczy, H. et al. Expression of an adhesion molecule and homing in B-cell chronic lymphocytic leukaemia: II. L-selectin expression mediated cell adhesion revealed by immobilized analogue carbohydrates in B-cell chronic lymphocytic leukaemia and monoclonal lymphocytosis of undetermined significance. Med Oncol. & Tumour Pharmacother. 10, 173–180 (1993). https://doi.org/10.1007/BF02989666

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02989666

Key words

Search

Navigation