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Medical Oncology and Tumor Pharmacotherapy

, Volume 10, Issue 4, pp 173–180 | Cite as

Expression of an adhesion molecule and homing in B-cell chronic lymphocytic leukaemia: II. L-selectin expression mediated cell adhesion revealed by immobilized analogue carbohydrates in B-cell chronic lymphocytic leukaemia and monoclonal lymphocytosis of undetermined significance

  • György Csanaky
  • János A. Vass
  • Hajna Losonczy
  • Matild Schmelczer
Article
  • 32 Downloads

Abstract

The L-selectin mediated adhesion of freshly isolated peripheral blood mononuclear cells (PBMCs) to phosphonomonoester core polysaccharide (PPME) and fucoidin derivatized gels was investigated in seven cases of monoclonal lymphocytosis of undetermined significance (B-MLUS) and 12 cases of chronic lymphocytic leukaemia: B-CLL, patients with peripheral lymphocytosis (LY-patients), lymph node enlargement (LN-patients) and splenomegaly (SM-patients). PBMCs isolated from the peripheral blood of 10 healthy donors served as controls. The binding to PPME and fucoidin correlated well(n = 19,P = 0. 01). Adhesion of PBMCs from B-MLUS and B-CLL showed a greater variability than controls. A higher number of cells, on average, bound to PPME and fucoidin derivatized polyacrylamide gels in B-MLUS than in B-CLL. However, the differences observed were not statistically significant. In four cases with B-CLL, the stimulatory effect of interferon-α on the function of L-selectin and some other accessory molecules was also studied. The increased binding of PBMCs to immobilized analogue molecules (PPME, fucoidin) and to high endothelial venules (HEVs) in thein vitro HEV-binding assay supports the notion that interferon-α not only increases the expression of the adhesion molecules, but also results in an enhanced adhesive function.

Key words

Selectins PPME fucoidin carbohydrate immobilization lymphocyte-endothelial interaction chronic lymphocytic leukaemia 

Abbreviations

HEV

high endothelial venule

B-CLL

B-cell chronic lymphocytic leukaemia

B-MLUS

monoclonal B lymphocytosis of undetermined significance

LN-patient

patient with lymph node enlargement

LY-patient

patient with peripheral lymphocytosis

SM-patient

patient with splenomegaly

PBMC

peripheral blood mononuclear cell

LECAM-1

lectin cell adhesion molecule-1

LAM-1

leucocyte adhesion molecule-1

GlyCAM

glyosylation dependent cell adhesion molecule

LFA-1

leucocyte functional antigen-1

ICAM-1

intercellular adhesion molecule-1

PPME

high-molecular-weight yeast phosphomannan core polysaccharide

PENT

phosphorylated pentasaccharide of mannose

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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • György Csanaky
    • 1
  • János A. Vass
    • 1
  • Hajna Losonczy
    • 2
  • Matild Schmelczer
    • 3
  1. 1.Department of PathologyUniversity Medical School of PécsPécsHungary
  2. 2.1st Department of Internal MedicineUniversity Medical School of PécsPécsHungary
  3. 3.2nd Department of Internal MedicineUniversity Medical School of PécsPécsHungary

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