Medical Oncology and Tumor Pharmacotherapy

, Volume 10, Issue 4, pp 173–180 | Cite as

Expression of an adhesion molecule and homing in B-cell chronic lymphocytic leukaemia: II. L-selectin expression mediated cell adhesion revealed by immobilized analogue carbohydrates in B-cell chronic lymphocytic leukaemia and monoclonal lymphocytosis of undetermined significance

  • György Csanaky
  • János A. Vass
  • Hajna Losonczy
  • Matild Schmelczer


The L-selectin mediated adhesion of freshly isolated peripheral blood mononuclear cells (PBMCs) to phosphonomonoester core polysaccharide (PPME) and fucoidin derivatized gels was investigated in seven cases of monoclonal lymphocytosis of undetermined significance (B-MLUS) and 12 cases of chronic lymphocytic leukaemia: B-CLL, patients with peripheral lymphocytosis (LY-patients), lymph node enlargement (LN-patients) and splenomegaly (SM-patients). PBMCs isolated from the peripheral blood of 10 healthy donors served as controls. The binding to PPME and fucoidin correlated well(n = 19,P = 0. 01). Adhesion of PBMCs from B-MLUS and B-CLL showed a greater variability than controls. A higher number of cells, on average, bound to PPME and fucoidin derivatized polyacrylamide gels in B-MLUS than in B-CLL. However, the differences observed were not statistically significant. In four cases with B-CLL, the stimulatory effect of interferon-α on the function of L-selectin and some other accessory molecules was also studied. The increased binding of PBMCs to immobilized analogue molecules (PPME, fucoidin) and to high endothelial venules (HEVs) in thein vitro HEV-binding assay supports the notion that interferon-α not only increases the expression of the adhesion molecules, but also results in an enhanced adhesive function.

Key words

Selectins PPME fucoidin carbohydrate immobilization lymphocyte-endothelial interaction chronic lymphocytic leukaemia 



high endothelial venule


B-cell chronic lymphocytic leukaemia


monoclonal B lymphocytosis of undetermined significance


patient with lymph node enlargement


patient with peripheral lymphocytosis


patient with splenomegaly


peripheral blood mononuclear cell


lectin cell adhesion molecule-1


leucocyte adhesion molecule-1


glyosylation dependent cell adhesion molecule


leucocyte functional antigen-1


intercellular adhesion molecule-1


high-molecular-weight yeast phosphomannan core polysaccharide


phosphorylated pentasaccharide of mannose


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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • György Csanaky
    • 1
  • János A. Vass
    • 1
  • Hajna Losonczy
    • 2
  • Matild Schmelczer
    • 3
  1. 1.Department of PathologyUniversity Medical School of PécsPécsHungary
  2. 2.1st Department of Internal MedicineUniversity Medical School of PécsPécsHungary
  3. 3.2nd Department of Internal MedicineUniversity Medical School of PécsPécsHungary

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