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Antioxidant protection against oxidant-induced damage in cultured gastric mucosal cells

  • Hideyuki Hiraishi
  • Noriaki Yajima
  • Norihisa Yamaguchi
  • Motoo Ishida
  • Yoshihisa Katoh
  • Takashi Harada
  • Akira Terano
  • Kevin J. Ivey
Basic Approach Recent Advances in Peptic Ulcer Research-Quality of Ulcer Healing

Summary

Gastric epithelium is exposed not only to oxidants generated within the lumen, but also to those produced by ischemia/reperfusion. This study examined the mechanism(s) of oxidant-induced injury to cultured rat gastric mucosal cells, and characterized the antioxidant profile of these cells. Hydrogen peroxide (H2O2), generated by glucose oxidase, damaged cells dose-dependently, as assessed by increased leakage of labeled51Cr. Glucose oxidase-induced damage was prevented by exogenous catalase (but not by exogenous Superoxide dismutase). Chelation of cellular iron with desferrioxamine or phenanthroline specifically protected cells against H2O2, whereas binding of extracellular iron with apotransferrin failed to. Disruption of the glutathione redox cycle at three independent sites rendered cells less resistant to H2O2, whereas inhibition of cellular catalase did not result in sensitization of cells to H2O2. In conclusion, (1) oxidant injury induced by extracellular H2O2 is mediated by intracellular iron; (2) extracellular Superoxide is not involved in the damaging process; and (3) the glutathione redox cycle plays a principal role in detoxifying H2O2 as a cellular antioxidant in cultured gastric mucosal cells.

Keywords

H202 Glucose Oxidase BCNU Desferrioxamine 51Cr Release 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used in this paper

BCNU

l,3-bis(chloroethyl)-l-nitrosourea

BSO

buthionine sulfoximine

DEM

diethyl maleate

GSH

reduced glutathione

GSSG

oxidized glutathione

H2O2

hydrogen peroxide

OH

hydroxyl radical

O2

Superoxide anion

SOD

Superoxide dismutase

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Copyright information

© The Japanese Society of Gastroenterology 1993

Authors and Affiliations

  • Hideyuki Hiraishi
    • 1
  • Noriaki Yajima
    • 1
  • Norihisa Yamaguchi
    • 1
  • Motoo Ishida
    • 1
  • Yoshihisa Katoh
    • 1
  • Takashi Harada
    • 1
  • Akira Terano
    • 2
  • Kevin J. Ivey
    • 3
  1. 1.Second Department of Internal MedicineDokkyo University School of MedicineTochigiJapan
  2. 2.Second Department of Medicine, Faculty of MedicineUniversity of TokyoTokyoJapan
  3. 3.Department of MedicineUniversity of CaliforniaIrvineUSA

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