Structural alterations of the ribosomal RNA genes in leukemic cells

  • Irene A. Smirnova


Cloned 6.7 kb EcoR1 fragment of mice rDNA was used as a hybridization probe for rDNA structure analysis in mice, rat and calf haemopoietic tumor and normal cells. EcoRl, BgIII and Pst1 restriction fragment length polymorphism (RFLP) was found in neoplastic rDNA and was not revealed in normal ones. The rRNA gene rearrangements were observed not only in spacer region but in coding sequences of the genes. Leukemic cells reveal also rDNA amplification.

A role of genetic rearrangements of rDNA for mechanisms of carcinogenesis is suggested.

Key Words

Leukemia rDNA Gene rearrangements Rauscher leukemia virus 



Rauscher ieukemic virus


ribosomal RNA


ribosomal DNA


enzootic bovine leukosis




0.15 M sodium chloride and 0.015 M sodium citrate


restriction fragment length polymorphism


nucleolar organizer region


sodium dodecyl sulfate


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Copyright information

© Humana Press Inc. 1992

Authors and Affiliations

  • Irene A. Smirnova
    • 1
  1. 1.Institute of Experimental Pathology, Oncology and RadiobiologyUkrainian Academy of SciencesKievUkraine

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