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Environmental Science and Pollution Research

, Volume 1, Issue 4, pp 229–233 | Cite as

Uptake and metabolism of 2,4,6-trinitrotoluene in higher plants

  • Elisabeth Görge
  • Sebastian Brandt
  • Dietrich Werner
Research Articles

Abstract

The fate of the explosive 2,4,6-TNT in plants is of major interest. Therefore, a method was developed to analyse TNT and derivatives in plant tissue. The method was utilized to investigate the uptake and metabolism of TNT inMedicago sativa andAllium schoenoprasum grown in hydroponic cultures containing TNT levels of 0.1 to 10 mg/1. Detectable concentrations of nitrotoluenes were significantly higher inAllium schoenoprasum than inMedicago sativa. The uptake of TNT in plants was directly related to the initial TNT level. The principal nitroaromatic components in roots and shoots of both plant species were identified as 4-ADNT and 2-ADNT in equal amounts, with substantially less TNT.

Keywords

Methylene Chloride Aromatic Amine Medicago Sativa Hydroponic Solution Nitrotoluenes 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature

  1. Amerkhanova, N. N.;R. P. Naumova: 2,4,6-Trinitrotoluene as a source of nutrition for bacteria. Microbiology47, 318 (1978)Google Scholar
  2. Broughton, W. J.;M. J. Dilworth: Control of leghaemoglobin in snake beans. Biochem. J. 125, 1075 (1971)Google Scholar
  3. Cataldo, D. A.; S. D. Harvey; R. J. Fellows; R. M. Bean; B. D. McVeety: An evaluation of the environmental fate and behavior of munitions material (TNT, RDX) in soil and plant systems. Pacific Northwest laboratories, Richland, Report AD-A223 546 (1989)Google Scholar
  4. Djerassi, L. S.;L. Vitany: Haemolytic episode in G6-PD deficient workers exposed to TNT. Brit. J. Ind. Med. 32, 54 (1975)Google Scholar
  5. Folsom, B. L.; J. C. Pennington; S. L. Teeter; M. R. Barton; J. A. Bright: Effects of soil pH and treatment level on persistence and plant uptake of 2,4,6-trinitrotoluene. Waterways Experiment Station, Corps of Engineers, Vicksburg, MS, Technical Report EL-88-22(1988)Google Scholar
  6. Grant, W. M.: Toxicology of the eye. Charles C. Thomas Publishers, Springfield Illinois 1986, p. 953Google Scholar
  7. Hathaway, J. A.: Trinitrotoluene — a review of reported dose-related effects providing documentation for a workplace standard. J. Occupa. Med.19, 341 (1977)Google Scholar
  8. Harvey, S. D.;R. J. Fellows;D. A. Cataldo;R. M. Bean: Analysis of 2,4,6-trinitrotoluene and its transformation products in soils and plant tissues by high-performance liquid chromatography. J. Chromato. 518, 361 (1990)CrossRefGoogle Scholar
  9. Koss, G.; A. Lommel; I. Ollroge; I. Tesseraux; R. Haas; A. D. Kappos: Zur Toxikologie der Nitrotoluole und weiterer Nitroaromaten aus rüstungsbedingten Altlasten. Bundesgesundhbl. 527 (1989)Google Scholar
  10. Osmon, J. L.;R. E. Klausmeier: The microbial degradation of explosives. Dev. Ind. Microbiol.14, 247 (1972)Google Scholar
  11. Palazzo, A. J.;D. C. Leggett: Effect and disposition of TNT in a terrestrial plant. J. Environ. Quail.15, 49 (1986)CrossRefGoogle Scholar
  12. Pennington, J. C.: Plant uptake of 2,4,6-trinitrotoluene, 4-amino-2,6-dinitrotoluene, and 2-amino-4,6-dinitrotoluene using 14C-labeled and unlabeled compounds. US Army Engineer Waterways Experiment Station, Miss Vicksburg: Technical Report EL-88-20th (1988)Google Scholar
  13. Preuss, A.;J. Fimpel;G. Diekert: Anaerobic transformation of 2,4,6-trinitrotoluene (TNT). Arch. Microbiol.159, 345 (1993)CrossRefGoogle Scholar
  14. SAS Institute: SAS/STAT Guide for Personal Computers, Version 6.03 Edition 1988Google Scholar
  15. Schott, C. D; E. G. Worthley: The toxicity of TNT and related wastes to an aquatic flowering plant,Lemna perpusilla. Edgewood Arsenal, Aberdeen Prov. Ground, MD. Techn. report 74016. AD-778158 (1974)Google Scholar
  16. Smock, L. A.;D. L. Stoneburger;J. R. Clark: The toxic effects of trinitrotoluene (TNT) and its primary degradation products on two species of algae and the fathead minnow. Water Res10, 537 (1976)CrossRefGoogle Scholar
  17. Spanggord, R. J.;T. Mill;T.-W. Chou;W. R. Maybe;J. H. Smith;S. Lee: Environmental fate studies on certain munition wastewater constituents. Final report. Phase II, Laboratory studies, SRI International, Menlo Park. CA (1980)Google Scholar
  18. Tucker, W. L.; W. L. Banwart; T. C. Granato; J. J. Hassett: Screening for plant tolerance to TNT. Report for USATHAMA by CERL and the University of Illinois (1989)Google Scholar
  19. V. Löw, E.;L. Kaminski;W. Neumeier;R. Haas;K. Steinbach: Mikrobieller Abbau von Nitroaromaten aus einer ehemaligen Sprengstoffproduktion. Forum Städte-Hygiene40, 347 (1989)Google Scholar
  20. Whipps, J. M.: Carbon economy. In:J. M. Lynch, The rhizosphere. John Wiley & Sons, Chichester 1990, pp. 59Google Scholar

Copyright information

© Ecomed Publishers 1994

Authors and Affiliations

  • Elisabeth Görge
    • 1
  • Sebastian Brandt
    • 1
  • Dietrich Werner
    • 1
  1. 1.Fachbereich BiologiePhilipps Universität MarburgMarburgGermany

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