Environmental Science and Pollution Research

, Volume 1, Issue 4, pp 205–208 | Cite as

On-line speciation of arsenical compounds in fish and mussel extracts by HPLC-ICP-MS

  • Sergio Caroli
  • Francesco La Torre
  • Francesco Petrucci
  • Nicola Violante
Research Articles


The separation and determination of sub-μmol/l levels of six environmentally significant As compounds was accomplished by means of high performance liquid chromatography (HPLC) combined online with inductively coupled plasma mass spectrometry (ICP-MS). The species of interest (arsenite and arsenate, monomethylarsonic acid, dimethylarsinic acid, arsenobetaine and arsenocholine) were quantified in fish and mussel extracts after separation on a Dionex AS7 column equipped with an on-guard AG7 column using a bicarbonate buffer as the mobile phase in a gradient mode. The species thus eluted were directly forwarded to the ICP-MS detector. The detection power of the overall system allows each As form to be determined at concentrations as low a 0.0013–0.0027 μmol 1−1.


High Performance Liquid Chromatography Inductively Couple Plasma Mass Spectrometry Arsenite Arsenic Speciation Arsenobetaine 


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Copyright information

© Ecomed Publishers 1994

Authors and Affiliations

  • Sergio Caroli
    • 1
  • Francesco La Torre
    • 1
  • Francesco Petrucci
    • 1
  • Nicola Violante
    • 1
  1. 1.Istituto Superiore di SanitàRomeItaly

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