Abstract
14C-TNT was used to quantify the uptake rate and metabolic turnover of TNT inPhaseolus vulgaris. Seventeen plants were analysed by a special cell fractionation method with polar and nonpolar solvents and enzymes. We obtained three cytoplasmic fractions and five cell wall derived fractions. The recovery rate was 72% as measured by liquid scintillation counting.14C partitioned almost in equal amounts with approximately 50% in the cytoplasm and in the cell wall. The majority of the TNT-metabolites are present in the cytoplasm as was shown by GC/ ECD and thin layer chromatography. The14C in the cell wall is bound probably resulting in long-term immobilisation of these metabolites. We conclude that plants may also be a model for nitroaromatic turnover and immobilisation in soil components.
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Sens, C., Scheidemann, P., Klunk, A. et al. Distribution of14C-TNT and derivatives in different biochemical compartments ofPhaseolus vulgaris . Environ. Sci. & Pollut. Res. 5, 202–208 (1998). https://doi.org/10.1007/BF02986402
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DOI: https://doi.org/10.1007/BF02986402