Effect of time and storage temperature on survival ofverticillium dahliae microsclerotia in dried potato stem tissue



Verticillium wilt (Vw) is a widespread and significant disease of potato (Solatium tuberosum). It is caused by the soil-borne fungiVerticillium dahliae andVerticillium albo-atrum. Host plant resistance is a promising method of Vw control. Culture-based methods that quantify the pathogen in host stem tissue are often used for Vw resistance screening. Either stem sap or dried stem tissue can be plated to measure pathogen populations in stems of plants that have been exposed to the pathogen. This study evaluated the effect of storage time and temperature on the survival ofV. dahliae microsclerotia in dried stem tissue. In both years of the study, the number of colony forming units (cfu) in stem samples did not change dramatically for the first six months in storage, regardless of storage temperature (-80 C, -20 C, 4 C, 23 C). After that, cfu means decreased sharply at all temperatures. Long-term survival of microsclerotia was lowest in samples stored at room temperature. These data indicate that stem samples can be stored for processing for several months. If processing is to occur after that, then samples should be stored at 4C.

Additional Key Words

disease resistance screening early dying disease Solanum tuberosum Verticillium wilt 

Literature Cited

  1. Butterfield EJ and JE DeVay. 1977. Reassessment of soil assays forVerticillium dahliae. Phytopathology 67:1073–1078.Google Scholar
  2. Dan H, ST Ali-Khan and J Robb. 2001. Use of quantitative PCR diagnostics to identify tolerance and resistance toVerticillium dahliae in potato. Plant Dis 85:700–705.CrossRefGoogle Scholar
  3. Davis JR. 1981. Verticillium wilt of potato in southeastern Idaho. University of Idaho, Current Information Series #564.Google Scholar
  4. Davis JR and OC Huisman. 2001. Verticillium wilt.In: WR Stevenson, R Loria, GD Franc and DP Weingartner (eds), Compendium of Potato Diseases, Ed 2. APS Press, St. Paul, pp 45–46.Google Scholar
  5. Davis JR, OC Huisman, DO Everson and AT Schneider. 2001. Verticillium wilt of potato: A model of key factors related to disease severity and tuber yield in southeastern Idaho. Amer J Potato Res 78:291–300.CrossRefGoogle Scholar
  6. Davis JR, JJ Pavek and DL Corsini. 1983. A sensitive method for quantifyingVerticillium dahliae colonization in plant tissue and evaluating resistance among potato genotypes. Phytopathology 73:1009–1014.CrossRefGoogle Scholar
  7. Hoyos GP, PJ Zambino and NA Anderson. 1991. An assay to quantify vascular colonization of potato byVerticillium dahliae. Am Potato J 68:727–742.CrossRefGoogle Scholar
  8. Isaac I and JAC Harrison. 1968. The symptoms and causal agents of early-dying disease (Verticillium wilt) of potatoes. Ann Appl Biol 61:231–244.CrossRefGoogle Scholar
  9. Plasencia J, R Jemmerson and EE Banttari. 1996. Production and characterization of monoclonal antibodies toVerticillium dahliae and development of a quantitative immunoassay for fungal biomass. Phytopathology 86:170–176.CrossRefGoogle Scholar
  10. Rowe RC. 1985. Potato early dying: A serious threat to the potato industry. Am Potato J 62:157–161.CrossRefGoogle Scholar
  11. Rowe RC, JR Davis, ML Powelson and DI Rouse. 1987. Potato early dying: Causal agents and management strategies. Plant Dis 71:482–489.CrossRefGoogle Scholar
  12. Sundaram S, J Plasencia and EE Bantarri. 1991. Enzyme-linked immunosorbent assay for detection ofVerticillium spp. using antisera produced toV. dahliae from potato. Phytopathology 81:1485–1489.CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  1. 1.Department of HorticultureUniversity of WisconsinUSA
  2. 2.Department of HorticultureUSDA/ARS and University of WisconsinUSA

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