, Volume 8, Issue 1, pp 27–37 | Cite as

The role of phenols in the pathogenicity ofBotrytis allii

  • G. Kritzman
  • I. Chet


The relationship betweenBotrytis allii and onion bulbs was studied in the greenhouse and the field. Germinating conidia or growing mycelium in nutrient solution, water, or in the plant tissue secreted pectinases and polyphenol oxidase. These pectinases were found to be inhibited by phenol compounds and their low oxidation products but not by polyphenols. Pretreatment of onion bulbs or seedlings with catechol (O-dihydroxybenzene), catechol + tannic acid, or phenyl-thiocarbamide increased the plant concentrations of phenols and decreased the disease incidence. Fungal polyphenol oxidase polymerizes the inhibitory plant phenols and neutralizes their effects. Phenylthiocarbamide inhibits polyphenol oxidase activity in the plant, resulting in inhibition of fungal attack.

Key Words

Interaction onion, bulb 


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  1. 1.
    Bateman, D.F. (1966) Hydrolytic and trans-eliminative degradation of pectic substances by extracellular enzymes ofFusarium solani f. phaseoli.Phytopathology 56: 238–244.PubMedCrossRefGoogle Scholar
  2. 2.
    Chet, I., Havkin, D. and Katan, J. (1978) The role of catechol in inhibition ofFusarium wilt.Phytopath. Z. 91: 60–66.CrossRefGoogle Scholar
  3. 3.
    Cole, J.S. (1956) Studies in the physiology of parasitism. XX, The pathogenicity ofBotrytis cinerea, Sclerotinia fructigena andSclerotinia laxa with special reference to the part played by pectic enzymes.Ann. Bot. (Lond.) 20: 15–38.Google Scholar
  4. 4.
    Cole, M. and Wood, R.K.S. (1961) Types of rot, rate of rotting and analysis of pectic substances in apples rotted by fungi.Ann. Bot. (Lond.) 25: 417–434.Google Scholar
  5. 5.
    Dische, Z. (1947) A new specific color reaction of hexuronic acids.J. biol. Chem. 167: 189–198.Google Scholar
  6. 6.
    Elson, L.A. and Morgan, W.T. (1933) A colorimetric method for the determination of glucosamine and chondrosamine.Biochem. J. 27: 1824–1828.PubMedGoogle Scholar
  7. 7.
    Hatfield, W.C., Walker, J.C. and Owen, J.H. (1948) Antibiotic substances in onion in relation to disease resistance.J. agric. Res. 77: 115–135.Google Scholar
  8. 8.
    Hudson, H.J. (1968) The ecology of fungi on plant remains above the soil.New Phytol. 67: 837–874.CrossRefGoogle Scholar
  9. 9.
    Jarvis, W.R. (1977)Botryotinia andBotrytis species. Taxonomy, physiology and pathogenicity.Monogr. Res. Branch Canada Dep. Agric. 15.Google Scholar
  10. 10.
    Kritzman, G. and Netzer, D. (1978) A selective medium for isolation and identification ofBotrytis spp. from soil and onion seed.Phytoparasitica 6: 3–7.CrossRefGoogle Scholar
  11. 11.
    Leonard, T.J. (1971) Phenoloxidase activity and fruiting body formation inSchizophyllum commune.J. Bacteriol. 106: 162–167.PubMedGoogle Scholar
  12. 12.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randell, R.J. (1951) Protein measurement with folin phenol reagent.J. biol. Chem. 193: 265–275.PubMedGoogle Scholar
  13. 13.
    Maas, J.L. and Powelson, R.L. (1972) Growth and sporulation ofBotrytis convoluta with various carbon and nitrogen sources.Mycologia 64: 897–903.PubMedCrossRefGoogle Scholar
  14. 14.
    Pliskanovskii, V.A. (1972) [The role of phenolic materials in the resistance of grape berries toBotrytis cinerea].Vinodel. Vinograd. SSSR 13: 48–53. (in Russian)Google Scholar
  15. 15.
    Pliskanovskii, V.A. and Zotov, V.V. (1971) [Factors determining the resistance of grape berries to gray mold].Skh. Biol. 6: 775–777. (in Russian)Google Scholar
  16. 16.
    Retig, N. and Chet, I. (1974) Catechol-induced resistance of phenols toFusarium wilt.Physiol. Pl. Path. 4: 469–475.CrossRefGoogle Scholar
  17. 17.
    Rizvanov, K. and Karadimcheva, B. (1973) [Fungicidal activity of anthocyanins in grapes].Lozar. Vinar. 21: 26–32. (in Russian)Google Scholar
  18. 18.
    Swain, T. and Hullis, W.E. (1959) Estimation of total phenols constituents ofPrunus domestica.J. Sci. Fd Agric. 10: 63–68.CrossRefGoogle Scholar
  19. 19.
    Verhoeff, K. and Liem, J.I. (1978) Presence of endo-Polygalacturonase in conidia ofBotrytis cinerea before and during germination.Phytopath. Z. 91: 110–115.CrossRefGoogle Scholar
  20. 20.
    Walker, J.C. and Lindegren, C.C. (1924) Further studies on the relation of onion scale pigmentation to disease resistance.J. agric. Res. 29: 507–514.Google Scholar
  21. 21.
    Walker, J.C., Lindegren, C.C. and Rachmann, F.M. (1925) Further studies on the toxicity of juice extracted from succulent onion scales.J. agric. Res. 30: 175–187.Google Scholar
  22. 22.
    Walker, J.C. and Link, K.P. (1935) Toxicity of phenolic compounds to certain onion bulb parasites.Bot. Gaz. 99: 468–484.CrossRefGoogle Scholar
  23. 23.
    Walker, J.C., Morell, S. and Foster, H.H. (1937) Toxicity of mustard oils and related sulfur compounds to certain fungi.Am. J. Bot. 24: 536–541.CrossRefGoogle Scholar
  24. 24.
    Walker, J.C., Owen, J.H. and Stahmann, M.A. (1950) Relative importance of phenols and volatile sulfides in disease resistance in the onion.Phytopathology 40: 30.Google Scholar
  25. 25.
    Zotov, V.V. and Pliskanovskii, V.A. (1973) [The nature of gray mold rot resistance in grapes].Mikol. Fitopatol. 7: 213–216. (in Russian)Google Scholar

Copyright information

© Springer Science + Business Media B.V. 1980

Authors and Affiliations

  • G. Kritzman
    • 1
  • I. Chet
    • 2
  1. 1.Div. of Plant Pathology, AROThe Volcani CenterBet Dagan
  2. 2.Dept. of Phytopathology and Microbiology, Faculty of AgricultureThe Hebrew University of JerusalemRehovot

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