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Archives of Pharmacal Research

, Volume 19, Issue 6, pp 520–528 | Cite as

Effects of forskolin on endogenous dopamine and acetylcholine release in rat neostriatal slices

  • Hwa-Jung Kim
Research Articles
  • 36 Downloads

Abstract

The involvement of the cyclic AMP (cAMP) effector system in the release of endogenous dopamine and acetylcholine from the rat neostriatum was assessed. Forskolin, an activator of adenylate cyclase, was used to enhance cAMP production, and the consequence of this enhancement on the spontaneous and potassium stimulated release of dopamine and a-cetylcholine was evaluated. Neostriatal slices were prepared from Fischer 344 rats and after a preincubation period the release of each endogenous neurotransmitter was measured from the same slice preparation. To measure acetylcholine release the slice acetylcholinesterase (AChE) activity was inhibited with physostigmine, but the release from slices with intact AChE activity was also determined (choline, instead of acetylcholine was detected in the medium). Under both conditions forskolin induced a significant dose-dependent increase in the potassium-evoked release of dopamine. In the same tissue preparations the release of neither acetylcholine (AChE inhibited) nor choline (AChE intact) was affected by forskolin. The results indicate that the cAMP second messenger system might be involved in neuronal mechanisms that enhance neostriatal dopamine release, but stimulation of this second messenger by forskolin does not further enhance neostriatal acetylcholine release.

Key words

cAMP effector system Forskolin Acetylcholine Dopamine Endogenous release Neostriatal slices 

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Copyright information

© The Pharmaceutical Society of Korea 1996

Authors and Affiliations

  • Hwa-Jung Kim
    • 1
  1. 1.College of PharmacyEwha Womans UniversitySeoulKorea

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