Journal of Biosciences

, Volume 25, Issue 1, pp 47–54 | Cite as

An enzyme immunoassay for detection of Japanese encephalitis virus-induced chemotactic cytokine

  • Aditi Singh
  • Rajesh Kulshreshtha
  • Asha Mathur


Japanese encephalitis virus (JEV) induces human peripheral blood monocytes to secrete a chemotactic cytokine [human macrophage-derived factor (hMDF)] which causes chemotaxis of neutrophils. The only known assay for hMDF cannot quantify its level in samples, so an enzyme immunoassay has been standardized for detection of hMDF and hMDF-specific antibodies in test samples. The reported enzyme linked immunosorbent assay (ELISA) was found to be sensitive (89%), specific (91%), accurate (92.2%) and reproducible and was able to detect a minimum concentration of 23 ng hMDF/ml in test samples. The chemotactic factor could be detected in JEV inoculated mouse sera and JEV infected culture fluids. Significant finding of the test was the detection of hMDF in sera of human cases of JE.


Enzyme immunoassay human macrophage-derived factor Japanese encephalitis virus 

Abbreviations used


Enzyme linked immunosorbent assay


Japanese encephalitis virus


human macrophage-derived factor


Hank’s balanced salt solution


cerebrospinal fluid


minimum essential medium


foetal calf serum


phosphate buffered saline


normal saline




horse raddish peroxiidase


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Copyright information

© Indian Academy of Sciences 2000

Authors and Affiliations

  1. 1.Postgraduate Department of MicrobiologyKG’s Medical CollegeLucknowIndia

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