An in vitro comparison of the oxidative effects of two structurally related cephalosporins on normal canine and human haemolysate
Two structurally related cephalosporins which produced haemolytic anaemia in the dog were compared in an in vitro test system which demonstrated oxidative changes in haemolysates prepared from canine and human erythrocytes. This system utilised lead acetate as an inhibitor of the antioxidant enzymes of the pentose phosphate pathway.
Increased methaemoglobin production was induced in haemolysates by both compounds but whereas lead acetate exacerbated the oxidant effect of one, a dihydroxy phenol cephalosporin, it did not exacerbate this effect of the other (cefoperazone), whose structure included a monohydroxy phenol side chain.
Under the in vitro conditions of the investigation, it was also evident that canine haemolysate showed much greater susceptibility to oxidation than human haemolysate.
KeywordsCephalosporins Haemolytic anaemia Lead acetate Methaemoglobin
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