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Chinese Journal of Cancer Research

, Volume 12, Issue 3, pp 183–187 | Cite as

Effects of IFN-α combined with il-6 on growth and expression of the genes related to cell-growth and apoptosis of bone marrow cells from patients with CML

  • Chen Han-chun 
  • Tang Li-jun 
  • Peng Xing-hua 
  • Luo Zhi-yong 
  • Luo Sai-qun 
  • Tan Wen-bin 
Advances in Brief
  • 22 Downloads

Abstract

Objective: To investigate the effects of interferon-α (IFN-α) and IFN-α combined with interleukin-6 (IL-6) on growth and expression of bcr-abl, bcl-2 and c-myc genes in the mononuclear cells (MNCs) from bone marrow (BM) of patients with chronic myelogenous leukemia (CML). Methods: MNCs were collected from BM of the patients with CML in chronic phase by centrifugation in lymphocyte separation medium and cultured in liquid with IFN-α (200U/ml) or IFN-α (200U/ml) plus IL-6 (100 ng/ml). The growing cells were counted every day. The expression levels of β-actin, bcr-abl, bcl-2 and c-myc genes in the MNCs incubated for 24 h were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and relatively quantitative analysis of the amplified fragments by optical density scanning for the bands on gel. Results: The cell growth was markedly suppressed by IFN-α but the degree of cell-growth inhibition was slightly decreased by IL-6 on the basis of IFN-α effect The expression of bcr-abl chimeric gene was intensely inhibited by IFN-α or IFN-α plus IL-6. The expression of bcl-2 gene was suppressed by either IFN-α or IFN-α plus IL-6, whereas that of c-myc gene was also inhibited by IFN-α but strongly elevated by IL-6 on the basis of IFN-α action. Conclusions: Both IFN-α and IFN-α plus IL-6 can inhibit the expression of anti-apoptosis gene such as bcr-abl and bcl-2 and regulate the expression of the gene related to cell proliferation and differentiation such as c-myc. Either IFN-α or IFN-α combined with IL-6 will serve as a trustful strategy of clinical treatment for CML.

Key words

Chronic myelogenous leukemia Combination therapy Interferon-a Interleukin-6 Gene expression Apoptosis 

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Copyright information

© Chinese Journal of Cancer Research 2000

Authors and Affiliations

  • Chen Han-chun 
    • 1
  • Tang Li-jun 
    • 1
  • Peng Xing-hua 
    • 1
  • Luo Zhi-yong 
    • 1
  • Luo Sai-qun 
    • 1
  • Tan Wen-bin 
    • 1
  1. 1.Research Center for Molecular BiologyHunan Medical UniversityChangshaChina

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