Abstract
Objective: To construct the eukaryotic expression vector that express human granulocyte-macrophage colony-stimulating factor (hGM-CSF) gene for making highly express in mammalian cells. Methods: Extract totally RNA from the induced human fetal lung (HFL) cell line. HGM-CSF cDNA was obtained by reverse transcription-polymerase chain reaction (RT-PCR), and then directionally subcloned into the HindIII and EcoRI site on the pcDNA3.1 plasmid, which was controlled by the CMV promoter, to form the recombinant expressing vector pcDNA3.1-GM-CSF. Results: The PCR amplification was identified and the sequence was analyzed, the results showed that hGM-CSF was properly inserted into the vector and the sequence was correct.
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This work is supported by the Natural Science Foundation of Fujian Province, China (No. C97067)
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Zheng, Qh., Zheng, Tr., Xie, Yq. et al. Construction of eukaryotic expression vector with granulocyte-macrophage colony-stimulating factor gene. Chin J Cancer Res 12, 125–127 (2000). https://doi.org/10.1007/BF02983437
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DOI: https://doi.org/10.1007/BF02983437