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Chinese Journal of Cancer Research

, Volume 12, Issue 2, pp 125–127 | Cite as

Construction of eukaryotic expression vector with granulocyte-macrophage colony-stimulating factor gene

  • Zheng Qiu-hong 
  • Zheng Tian-rong 
  • Xie Yun-qing 
  • Lu Lin 
  • Chen Hui 
Basic Investigation

Abstract

Objective: To construct the eukaryotic expression vector that express human granulocyte-macrophage colony-stimulating factor (hGM-CSF) gene for making highly express in mammalian cells. Methods: Extract totally RNA from the induced human fetal lung (HFL) cell line. HGM-CSF cDNA was obtained by reverse transcription-polymerase chain reaction (RT-PCR), and then directionally subcloned into the HindIII and EcoRI site on the pcDNA3.1 plasmid, which was controlled by the CMV promoter, to form the recombinant expressing vector pcDNA3.1-GM-CSF. Results: The PCR amplification was identified and the sequence was analyzed, the results showed that hGM-CSF was properly inserted into the vector and the sequence was correct.

Key words

Human granulocyte-macrophage colony-stimulating factor (hGM-CSF) Reverse transcription and polymerse chain reaction (RT-PCR) Eukaryotic expression 

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Copyright information

© Chinese Journal of Cancer Research 2000

Authors and Affiliations

  • Zheng Qiu-hong 
    • 1
  • Zheng Tian-rong 
    • 1
  • Xie Yun-qing 
    • 1
  • Lu Lin 
    • 1
  • Chen Hui 
    • 1
  1. 1.Department of Tumor Molecular BiologyFujian Tumor HospitalFuzhouChina

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