Advertisement

Phytoparasitica

, Volume 6, Issue 1, pp 3–7 | Cite as

A selective medium for isolation and identification ofBotrytis spp. from Soil and onion Seed

  • G. Kritzman
  • D. Netzer
Article

Abstract

The population ofBotrytis allii in either naturally or artificially infested soils was selectively measured by the soil dilution plate count procedure on a developed synthetic medium supplemented with tannic acid, fungicides, antibiotics and Cu++ions. Conversion of tannic acid into a dark brown pigment was related to the activity of extracellular polyphenoloxidase produced by the fungus. Thus, brown-pigmented colonies were recognized asBotrytis spp. The same medium was used for detecting the presence of the fungus on onion seed.

Key Words

Botrytis allii tannic acid selective inhibition 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Clark, C.A. andLorbeer, J.W. (1975) The role of phenols in Botrytis brown stain of onion.Phytopathology65: 338–341.CrossRefGoogle Scholar
  2. 2.
    Komada, H. (1972) Studies on the method for selective isolation ofFusarium oxysporum from natural soil.Bull. Tokai-Kinki. natn. agric. Exp. Stn23: 144–178.Google Scholar
  3. 3.
    Lorbeer, J.W. andTichelaar, G.M. (1970) A selective medium for the assay ofBotrytis allii in organic and mineral soils.Phytopathology60: 1301 (Abstr.).Google Scholar
  4. 4.
    Lyr, H. (1958) Uber den Nachweis von Oxydasen und Peroxydasen bei hoheren Pilzen und die Bedeutung dieser Enzyme für die Bavendamm Reaktion.Planta, Bed.50: 359–370.CrossRefGoogle Scholar
  5. 5.
    Maude, R.B. andPresly, A.H. (1974) Neck rot of onions.A. Rep. Nat. Veg. Res. Stn, Wellesbourne1973: 94–95.Google Scholar
  6. 6.
    Maude, R.B. andPresly, A.H. (1977) Neck rot (Botrytis allii) of bulb onions. 1. Seed-borne infection and its relationship to the disease in the onion crop.Ann. appl Biol86: 163–180.CrossRefGoogle Scholar
  7. 7.
    Sneh, B., Netzer, D. andKrikun, J. (1974) Isolation and identification ofPyrenochaeta terrestris from soil dilution plates.Phytopathology64: 275–276.Google Scholar
  8. 8.
    Walker, J.C. (1972) Diseases of Vegetable Crops. McGraw-Hill, New York, N.Y.Google Scholar

Copyright information

© Springer Science + Business Media B.V. 1978

Authors and Affiliations

  • G. Kritzman
  • D. Netzer
    • 1
  1. 1.Div. of Plant PathologyARO, The Volcani CenterBet Dagan

Personalised recommendations