, Volume 6, Issue 1, pp 3–7 | Cite as

A selective medium for isolation and identification ofBotrytis spp. from Soil and onion Seed

  • G. Kritzman
  • D. Netzer


The population ofBotrytis allii in either naturally or artificially infested soils was selectively measured by the soil dilution plate count procedure on a developed synthetic medium supplemented with tannic acid, fungicides, antibiotics and Cu++ions. Conversion of tannic acid into a dark brown pigment was related to the activity of extracellular polyphenoloxidase produced by the fungus. Thus, brown-pigmented colonies were recognized asBotrytis spp. The same medium was used for detecting the presence of the fungus on onion seed.

Key Words

Botrytis allii tannic acid selective inhibition 


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Copyright information

© Springer Science + Business Media B.V. 1978

Authors and Affiliations

  • G. Kritzman
  • D. Netzer
    • 1
  1. 1.Div. of Plant PathologyARO, The Volcani CenterBet Dagan

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