A selective medium for isolation and identification ofBotrytis spp. from Soil and onion Seed
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The population ofBotrytis allii in either naturally or artificially infested soils was selectively measured by the soil dilution plate count procedure on a developed synthetic medium supplemented with tannic acid, fungicides, antibiotics and Cu++ions. Conversion of tannic acid into a dark brown pigment was related to the activity of extracellular polyphenoloxidase produced by the fungus. Thus, brown-pigmented colonies were recognized asBotrytis spp. The same medium was used for detecting the presence of the fungus on onion seed.
Key WordsBotrytis allii tannic acid selective inhibition
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