, Volume 25, Issue 4, pp 305–318 | Cite as

Antagonistic activity of Bacteria from the chickpea rhizosphere againstFusarium Oxysporum f. sp.Ciceris

  • Bianca B. Landa
  • Ana Hervás
  • Wagner Bettiol
  • Rafael M. Jiménez-Díaz


The antagonistic activity againstin vitro growth ofFusarium oxysporum f. sp.ciceris was determined for 74 bacterial isolates obtained from the rhizosphere of chickpeas grown in two field soils with different histories of Fusarium wilt, and for seven isolates ofPseudomonas spp. from culture collections. Twenty-four isolates ofBacillus spp. andPseudomonas chlororaphis 30-84 showed a strong antagonism against three races (0, 1 and 5) ofF. o. ciceris tested. Three selectedBacillus isolates andP. chlororaphis 30-84 were further tested against 30 isolates of races 0, 1 and 5 ofF. o. ciceris, races 0, 1 and 2 ofF. o. melonis, F. o. phaseoli and nonpathogenicF. oxysporum. Bacillus isolates differed in their antagonistic activity and were less inhibitory to mycelial growth ofF. o. ciceris than to that of other fungal isolates. Furthermore, the extent of growth inhibition ofF. o. ciceris was influenced both by bacterial isolates and by race of the pathogen. Cell-free culture filtrates of fourBacillus isolates inhibited conidial germination and hyphal growth ofF. o. ciceris and nonpathogenicF. oxysporum. Joint seed+soil treatment with some selected antagonisticBacillus spp. isolates suppressed disease caused by the highly virulentF. o. ciceris race-5 in cv. ICCV 4 and cv. PV 61 chickpeas. However, the degree of protection was influenced by the host genotype and the inoculum concentration of the pathogen.

Key words

Antagonism Bacillus spp. biological control chickpea Cicer arietinum Fusarium wilt fluorescent pseudomonads 


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Copyright information

© Springer Science + Business Media B.V. 1997

Authors and Affiliations

  • Bianca B. Landa
    • 1
  • Ana Hervás
    • 1
  • Wagner Bettiol
    • 2
  • Rafael M. Jiménez-Díaz
    • 1
  1. 1.Dept. de Protectión de Cultivos, Inst. de Agricultura SostenibleConsejo Superior de Investigaciones CientíficasCórdobaSpain
  2. 2.CNPMA/EMBRAPAJaguaricina SPBrazil

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