Skip to main content
Log in

Note: Pyrethroid resistance and possible involvement of glutathioneS-transferases inHelicoverpa armigera from Turkey

  • Entomology
  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

An introductory study was conducted to investigate the pyrethroid resistance ofHelicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) strains in Turkey, collected from cotton fields in the Adana and Antalya provinces, through two different synthetic pyrethroid insecticides: lambda-cyhalothrin and esfenvalerate. In addition, the roles of glutathioneS-transferases (GSTs) in this resistance mechanism were analyzed. It was found that whereas resistance ratios for lambda-cyhalothrin (LD50 levels) were 3- and 98-fold increased in the Adana and Antalya strains, respectively, esfenvalerate ratios were 3.3- and 92.3-fold increased in the Adana and Antalya strains, respectively, with respect to the susceptible strain. Furthermore, Adana and Antalya strains showed 2.4- and 2.9-fold higher GST activities than the susceptible strain, respectively. In the Antalya field strain, the minor increase in GST activity compared with the resistance levels implies that GSTs may be not greatly involved in this resistance. It also provides evidence that they could not be the only metabolic mechanism responsible for resistance to lambda-cyhalothrin and esfenvalerate inH. armigera from Turkey.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Anon. (1970) Standard method for detection of insecticide resistance inHeliothis zea (Boddie) andH. virescens (F.).Bull. Entomol. Soc. Am. 16:147–153.

  2. Clark, A.G., Shamaan, N.A., Sinclair, M.D. and Dauterman, W.C. (1986) Insecticide metabolism by multiple glutathioneS-transferases in two strains of the housefly,Musca domestica (L.).Pest. Biochem. Physiol. 25:169–175.

    Article  CAS  Google Scholar 

  3. Fournier, D., Bride, J.M., Poire, M., Berge, J.B. and Plapp, F.W. Jr. (1992) Insect glutathioneS-transferases. Biochemical characteristics of the major forms from houseflies susceptible and resistant to insecticides.J. Biol. Chem. 267:1840–1845.

    PubMed  CAS  Google Scholar 

  4. Gunning, R.V., Moores, G.D. and Devonshire, A.L. (1996) Esterases and esfenvalerate resistance in AustralianHelicoverpa armigera (Hübner) (Lepidoptera: Noctuidae).Pest. Biochem. Physiol. 54:12–23.

    Article  CAS  Google Scholar 

  5. Gunning, R.V., Moores, G.D. and Devonshire, A.L. (1999) Esterase inhibitors synergise the toxicity of pyrethroids in AustralianHelicoverpa armigera (Hübner) (Lepidoptera: Noctuidae).Pest. Biochem. Physiol. 63:50–62.

    Article  CAS  Google Scholar 

  6. Habig, W.H., Pabst, M.J. and Jakoby, W.B. (1974) GlutathioneS-transferases: the first enzymatic step in mercapturic acid formation.J. Biol. Chem. 249:7130–7139.

    PubMed  CAS  Google Scholar 

  7. Kostaropoulos, I., Papadopoulos, A.I., Metaxakis, A., Boukouvala, E. and Papadopoulou-Maurkidou, E. (2001) GlutathioneS-transferase in the defence against pyrethroids in insects.Insect Biochem. Mol. Biol. 31:313–319.

    Article  PubMed  CAS  Google Scholar 

  8. LeOra Software (1994) POLO-PC: A User’s Guide to Probit or Logit Analysis. LeOra Software, Berkeley, CA, USA.

    Google Scholar 

  9. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randal, R.J. (1951) Protein measurement with the folin phenol reagent.J. Biol. Chem. 248:265–275.

    Google Scholar 

  10. Martin, T., Chandre, F., Ochou, O.G., Vaissayre, M. and Fournier, D. (2002) Pyrethroid resistance mechanisms in the cotton bollwormHelicoverpa armigera (Lepidoptera: Noctuidae) from West Africa.Pestic. Biochem. Physiol. 74:17–26.

    Article  Google Scholar 

  11. McCaffery, A.R. (1998) Resistance to insecticides in heliothine Lepidoptera: a global view.Philos. Trans. R. Soc. Lond. B Biol. Sci. 353:1735–1750.

    Article  CAS  Google Scholar 

  12. Parkes, T.L, Hilliker, A.J. and Phillips, J.P. (1993) Genetic and biochemical analysis of glutathioneS-transferases in the oxygen defence system ofDrosophila melanogaster.Genome 36:1007–1014.

    Article  PubMed  CAS  Google Scholar 

  13. Qiu, X.-H. and Leng, X.-F. (2000) The tissue distribution of monooxygenase activities of the cotton bollworm,Helicoverpa armigera.Acta Ecol. Sin. 20:299–303 (Chinese, with English abstr.).

    Google Scholar 

  14. Ranson, H., Prapanthadara, L. and Hemingway, J. (1997) Cloning and characterization of two glutathioneS-transferases from a DDT resistant strain ofAnopheles gambiae.Biochem. J. 324:97–102.

    PubMed  CAS  Google Scholar 

  15. Salinas, A.E. and Wong, M.G. (1999) GlutathioneS-transferases — a review.Curr. Med. Chem. 6:279–309.

    PubMed  CAS  Google Scholar 

  16. Sawicki, R., Singh, S.P., Mondal, A.K., Benes, H. and Zimniak, P. (2003) Cloning, expression and biochemical characterization of one Epsilon-class (GST-3) and ten Delta-class (GST-1) glutathioneS-transferases fromDrosophila melanogaster, and identification of additional nine members of the Epsilon class.Biochem. J. 370:661–669.

    Article  PubMed  CAS  Google Scholar 

  17. Vontas, J.G., Small, G.J. and Hemingway, J. (2001) GlutathioneS-transferases as antioxidant defence agents confer pyrethroid resistance inNilaparvata lugens.Biochem. J. 357:65–72.

    Article  PubMed  CAS  Google Scholar 

  18. Wang, J.Y., McCommas, S. and Syvanen, M. (1991) Molecular cloning of a glutathioneS-transferase overproduced in an insecticide-resistant strain of the housefly (Musca domestica).Mol. Gen. Genet. 227:260–266.

    Article  PubMed  CAS  Google Scholar 

  19. Wu, Y. and Shen, J. (1996) Characteristics of fenvalerate resistance inHelicoverpa armigera (Hübner) from China.Resistant Pest Manag. Newsl. 8(1):25–27.

    CAS  Google Scholar 

  20. Yang, E., Yang, Y., Wu, S. and Wu, Y. (2005) Relative contribution of detoxifying enzymes to pyrethroid resistance in a resistant strain ofHelicoverpa armigera.J. Appl. Entomol. 129:521–525.

    Article  CAS  Google Scholar 

  21. Yang, Y., Wu, Y., Chen, S., Devine, G.J., Denholm, I., Jewess, P.et al. (2004) The involvement of microsomal oxidases in pyrethroid resistance inHelicoverpa armigera from Asia.Insect Biochem. Mol. Biol. 34:763–773.

    Article  PubMed  CAS  Google Scholar 

  22. Yu, S.J. (1995) Tissue-specific expression of glutathione transferase isozymes in fall armyworm larvae.Pestic. Biochem. Physiol. 53:164–171.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Sakine Ugurlu.

Additional information

http://www.phytoparasitica.org posting Nov. 16, 2006.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ugurlu, S., Konus, M., Isgor, B. et al. Note: Pyrethroid resistance and possible involvement of glutathioneS-transferases inHelicoverpa armigera from Turkey. Phytoparasitica 35, 23–26 (2007). https://doi.org/10.1007/BF02981058

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02981058

Key words

Navigation