, Volume 32, Issue 1, pp 61–65 | Cite as

Seed transmission ofFusarium oxysporum f.sp.lactucae

  • Angelo Garibaldi
  • Giovanna Gilardi
  • Maria Lodovica Gullino


Twenty-seven seed samples belonging to the lettuce cultivars most frequently grown in Lombardy (northwestern Italy), in an area severely affected by Fusarium wilt of lettuce, were assayed for the presence ofFusarium oxysporum on a Fusarium-selective medium. Isolations were carried out on subsamples of seeds (500 to 1500) belonging to the same seed lots used for sowing, and either unwashed or disinfected in 1% sodium hypochloride. The pathogenicity of the isolates ofF. oxysporum obtained was tested in four trials carried out on lettuce cultivars of the butterhead type, very susceptible to Fusarium wilt. Nine of the 27 samples of seeds obtained from commercial seed lots used for sowing in fields affected by Fusarium wilt were contaminated byF. oxysporum. Among the 16 isolates ofF. oxysporum obtained, only one was isolated from disinfected seeds. Three of the isolates were pathogenic on the tested cultivars of lettuce, exhibiting a level of pathogenicity similar to that of the isolates ofF. oxysporum f.sp.lactucae obtained from infected wilted plants in Italy, USA and Taiwan, used as comparison. The results obtained indicate that lettuce seeds are a potential source of inoculum for Fusarium wilt of lettuce. The possibility of isolatingF. oxysporum f.sp.lactucae, although from a low percent of seeds, supports the hypothesis that the rapid spread of Fusarium wilt of lettuce observed recently in Italy is due to the use of infected propagation material. Measures for prevention and control of the disease are discussed.

Key Words

Lettuce Fusarium wilt disease management 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Chiocchetti, A., Sciaudone, L., Durando, F., Garibaldi, A. and Migheli, Q. (2001) PCR detection ofFusarium oxysporum f.sp.basilici on basil.Plant Dis. 85:607–611.CrossRefGoogle Scholar
  2. 2.
    Forsberg, G. (2001) Heat sanitation of cereal seeds with a new, efficient, cheap and environmentally friendly Seed Treatment: Challenges and Opportunities.Proc. BCPC Symp. no. 76, pp. 111–116.Google Scholar
  3. 3.
    Fujinaga, M., Ogiso, H., Tuchiya, N., Saito, H., Yamanaka, S., Nozue, al. (2003) Race 3, a new race ofFusarium oxysporum f.sp.lactucae determined by a differential system with commercial cultivars.J. Gen. Plant Pathol. 69:23–28.CrossRefGoogle Scholar
  4. 4.
    Garibaldi, A., Gilardi, G. and Gullino, M.L. (2002) First report ofFusarium oxysporum on lettuce in Europe.Plant Dis. 86:1052.CrossRefGoogle Scholar
  5. 5.
    Garibaldi, A., Gilardi, G. and Gullino, M.L. (2004) Varietal resistance of lettuce toFusarium oxysporum f.sp.lactucae. Crop Prot. 24 (in press).Google Scholar
  6. 6.
    Grube, R.C., Ryder, E.J., Koibe, S.T., McCreight, J.M. and Wintermantel, W.M. (2003) Breeding for resistance to new and emerging lettuce diseases in California.Proc. Eucarpia Meeting of Leafy Genetics and Breedings (Noordwijkerhout, the Netherlands), pp. 37–42.Google Scholar
  7. 7.
    Huang, J.H. and Lo, C.T. (1998) Wilt of lettuce caused byFusarium oxysporum in Taiwan.Plant Pathol. Bull. 7:150–153.Google Scholar
  8. 8.
    Hubbard, J.C. and Gerik, J.S. (1993) A new disease of lettuce incited byFusarium oxysporum f.sp.lactucum forma specialis nov.Plant Dis. 77:750–754.Google Scholar
  9. 9.
    Komada, H. (1975) Development of a selective medium for quantitative isolation ofFusarium oxysporum from natural soil.Rev. Plant Prot. Res. 8:114–125.Google Scholar
  10. 10.
    Matuo, T. and Motohashi, S. (1967) OnFusarium oxysporum f.sp.lactucae n.f. causing root rot of lettuce.Trans. Mycol. Soc. Jpn. 8:13–15.Google Scholar
  11. 11.
    Ryder, R.J. (1998) Lettuce, Endive and Chicory. CABI Publishing, Wallingford, UK.Google Scholar

Copyright information

© Springer Science + Business Media B.V. 2004

Authors and Affiliations

  • Angelo Garibaldi
  • Giovanna Gilardi
  • Maria Lodovica Gullino
    • 1
  1. 1.Centre of Competence for Innovation in the Agro-Environmental Sector (AGRINNOVA)University of TorinoGrugliascoItaly

Personalised recommendations