Note: Anti-viral activity ofAilanthus altissima crude extract onRice stripe virus in rice suspension cells
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Ailanthus altissima extracts have previously been shown to have potent anti-fungal, anti-inflammation, anti-fertility, plant-growth-regulatory and insecticidal activities. This study demonstrated the anti-viral activity ofA. altissima crude extract againstRice stripe virus (RSV) in rice suspension cells, determined by inhibiting RSV crude protein transcript and expression using real-time reverse transcription polymerase chain reaction and Western blotting, respectively. TheA. altissima crude extract showed a strong inhibitory activity against RSV at a medium effective concentration of 0.55 µg ml−1. No significant cytotoxicity in rice suspension cells was shown.A. altissima crude extract would likely be a valuable antiviral agent for control of RSV.
Key wordsInhibitory activity Laodelphax striatellus real-time RT-PCR assay rice stripe disease CP transcript and expression
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