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Phytoparasitica

, Volume 36, Issue 4, pp 405–408 | Cite as

Note: Anti-viral activity ofAilanthus altissima crude extract onRice stripe virus in rice suspension cells

  • J. G. Yang
  • Y. G. Dang
  • G. Y. Li
  • L. J. Guo
  • W. T. Wang
  • Q. W. Tan
  • Q. Y. Lin
  • Z. J. Wu
  • L. H. Xie
Virology

Abstract

Ailanthus altissima extracts have previously been shown to have potent anti-fungal, anti-inflammation, anti-fertility, plant-growth-regulatory and insecticidal activities. This study demonstrated the anti-viral activity ofA. altissima crude extract againstRice stripe virus (RSV) in rice suspension cells, determined by inhibiting RSV crude protein transcript and expression using real-time reverse transcription polymerase chain reaction and Western blotting, respectively. TheA. altissima crude extract showed a strong inhibitory activity against RSV at a medium effective concentration of 0.55 µg ml−1. No significant cytotoxicity in rice suspension cells was shown.A. altissima crude extract would likely be a valuable antiviral agent for control of RSV.

Key words

Inhibitory activity Laodelphax striatellus real-time RT-PCR assay rice stripe disease CP transcript and expression 

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References

  1. 1.
    Chu, C.C., Wang, C.S., Sun, C.S., Hus, C., Ying, K.C., Chu, C.Y.et al. (1975) Establishment of an efficient medium for another culture of rice through comparative experiments on the nitrogen sources.Sci. Sin. 18:659–668.Google Scholar
  2. 2.
    Jain, M., Nijhawan, A., Tyagi, A.K. and Khurana, J.P. (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR.Biochem. Biophys. Res. Commun. 345:646–651.PubMedCrossRefGoogle Scholar
  3. 3.
    Lieberherr, D., Thao, N.N., Nakashima, A., Umemura, K., Kawasaki, T. and Shimamoto, K. (2005) A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice.Plant Physiol. 138:1644–1652.PubMedCrossRefGoogle Scholar
  4. 4.
    Lin, Q.Y., Xie, L.H., Zhou, Z.J., Xie, L.Y. and Wu, Z.J. (1990) Studies on rice stripe. I. Distribution of and losses caused by the disease.J. Fujian Agric. Univ. 19:421–425 (Chinese, with English abstract).Google Scholar
  5. 5.
    Mackay, I.M., Arden, K.E. and Nitsche, A. (2002) Real-time PCR in virology.Nucl. Acids Res. 30:1292–1305.PubMedCrossRefGoogle Scholar
  6. 6.
    Ravichandran, V., Suresh-Sathishkumar, M.N., Elango, K. and Srinivasan, R. (2007) Antifertility activity of hydroalcoholic extract ofAilanthus excelsa (Roxb): An ethnomedicine used by tribals of Nilgiris region in Tamilnadu.J. Ethnopharmacol. 112:189–191.PubMedCrossRefGoogle Scholar
  7. 7.
    Tamura, S., Fukamiya, N., Okano, M., Koyama, J., Koike, K., Tokuda, H.et al. (2003) Three new quassinoids, ailantinol E, F, and G, fromAilanthus altissima.Chem. Pharmacol. Bull. 51:385–389.CrossRefGoogle Scholar
  8. 8.
    Toriyama, S. (1982) Characterization of rice stripe virus: a heavy component carrying infectivity.J. Gen. Virol. 61:187–195.CrossRefGoogle Scholar
  9. 9.
    Toriyama, S. (1986) Rice stripe virus: prototype of a new group of viruses that replicate in plants and insects.Microbiol Sci. 3:347–351.PubMedGoogle Scholar
  10. 10.
    Tsao, R., Romanchuk, F.E., Peterson, C.J. and Coats, J.R. (2002) Plant growth regulatory effect and insecticidal activity of the extracts of the Tree of Heaven (Ailanthus altissima L.).BMC Ecol. 2:1–6.PubMedCrossRefGoogle Scholar
  11. 11.
    Yang, W., Wang, X., Wang, S., Yie, Y. and Tien, P. (1996) Infection and replication of a planthopper transmitted virus: rice stripe virus in rice protoplasts.J. Virol. Methods 59:57–60.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media B.V. 2008

Authors and Affiliations

  • J. G. Yang
    • 1
  • Y. G. Dang
    • 1
  • G. Y. Li
    • 1
  • L. J. Guo
    • 1
  • W. T. Wang
    • 1
  • Q. W. Tan
    • 1
  • Q. Y. Lin
    • 1
  • Z. J. Wu
    • 1
  • L. H. Xie
    • 1
  1. 1.Institute of Plant Virology and Key Lab of Plant Virology of Fujian ProvinceFujian Agriculture and Forestry UniversityFuzhouP.R. China

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