Advertisement

Phytoparasitica

, 11:187 | Cite as

Complementary bacterial enrichment techniques for the detection ofPseudomonas syringae pv.tomato andXanthomonas campestris pv.vesicatoria in infested tomato and pepper seeds

  • Y. Bashan
  • I. Assouline
Article

Abstract

A scheme for routine seed testing forXanthomonas campestris pv.vesicatoria andPseudomonas syringae pv.tomato in pepper and tomato seeds was developed. The scheme is based on different bacterial enrichment techniques. As few as 1000 and 10–100 colony forming units per gram of seeds were detected using a liquid enrichment technique or leaf enrichment technique, respectively. Relatively large amounts of saprophytes on the seed surfaces did not interfere with the detection of the pathogens.

Key Words

Bacterial speck of tomato bacterial scab of pepper seedborne pathogens phytopathogenic bacteria 

References

  1. 1.
    Bashan, Y., Okon, Y. and Henis, Y. (1978) Infection studies ofPseudomonas tomato, causal agent of bacterial speck of tomato.Phytoparasitica 6: 135–143.Google Scholar
  2. 2.
    Chambers, S.C. and Merriman, P.R. (1975) Perennation and control ofPseudomonas tomato in Victoria.Aust. J. agric. Res. 26: 657–663.CrossRefGoogle Scholar
  3. 3.
    Crossan, D.F. and Morehart, A.L. (1964) Isolation ofXanthomonas vesicatoria from tissues ofCapsicum annuum.Phytopathology 54: 358–359.Google Scholar
  4. 4.
    Dempsey, A.W. and Walker, J.T. (1973) Efficacy of calcium and sodium hypochlorite for seed treatment of pepper.HortScience 8: 328–329.Google Scholar
  5. 5.
    Devash, Y., Okon, Y. and Henis, Y. (1980) Survival ofPseudomonas tomato in soil and seeds.Phytopath. Z. 99: 175–185.CrossRefGoogle Scholar
  6. 6.
    King, E.O., Ward, M.K. and Raney, D.E. (1954) Two simple media for the demonstration of pyocyanin and fluorescin.J. Lab. Clin. Med. 44: 301–307.PubMedGoogle Scholar
  7. 7.
    Lelliott, R.A., Billing, E. and Hayward, A.C. (1966) A determinative scheme for the fluorescent plant pathogenic pseudomonads.J. appl. Bact. 29: 470–489.Google Scholar
  8. 8.
    Lewis, G.D. and Brown, D.H. (1961) Studies on the overwintering ofXanthomonas vesicatoria in New Jersey.Phytopathology 51: 557 (abstr.).Google Scholar
  9. 9.
    Neergaard, P. (1977) Seed Pathology. Macmillan Press, London.Google Scholar
  10. 10.
    Schaad, N.W. (1982) Detection of seedborne bacterial plant pathogens.Pl. Dis. 66: 885–890.Google Scholar
  11. 11.
    Schaad, N.W. and Donaldson, R.C. (1980) Comparison of two methods for detection ofXanthomonas campestris in infected crucifer seeds.Seed Sci. Technol. 8: 383–391.Google Scholar
  12. 12.
    Schaad, N.W. and Kendrick, R. (1975) A qualitative method of detectingXanthomonas campestris in crucifer seeds.Phytopathology 65: 1034–1036.CrossRefGoogle Scholar
  13. 13.
    Schaad, N.W. and White, W.C. (1974) A selective medium for soil isolation and enumeration ofXanthomonas campestris.Phytopathology 64: 876–877.Google Scholar
  14. 14.
    Shackleton, D.A. (1962) A method for the detection ofXanthomonas campestris (Pammel 1895) Dowson 1939, in Brassica seed.Nature, Lond. 193: 78.CrossRefGoogle Scholar
  15. 15.
    Sharon, E., Okon, Y., Bashan, Y. and Henis, Y. (1982) Detached leaf enrichment: A method for detecting small numbers ofPseudomonas syringae pv.tomato andXanthomonas campestris pv.vesicatoria in seeds and symptomless leaves of tomato and pepper.J. appl. Bact. 53: 371–377.Google Scholar
  16. 16.
    Srinivasan, M.C., Neergaard, P. and Mathur, S.B. (1973) A technique for detection ofXanthomonas campestris in routine seed health testing of crucifers.Seed Sci. Technol. 1: 853–859.Google Scholar
  17. 17.
    Taylor, J. (1962) The estimation of numbers of bacteria by ten-fold dilution series.J. appl. Bact. 25: 54–61.Google Scholar
  18. 18.
    Wilson, E.E., Zeitoun, F.M. and Fredrickson, D.L. (1967) Bacterial phloem canker, a new disease of Persian walnut trees.Phytopathology 57: 618–621.Google Scholar
  19. 19.
    Yunis, H., Bashan, Y., Okon, Y. and Henis, Y. (1980) Two sources of resistance to bacterial speck of tomato caused byPseudomonas tomato.Pl. Dis. 64: 851–852.Google Scholar
  20. 20.
    Yunis, H., Bashan, Y., Okon, Y. and Henis, Y. (1980) Weather dependence, yield losses and control of bacterial speck of tomato caused byPseudomonas tomato.Pl. Dis. 64: 937–939.Google Scholar

Copyright information

© Springer Science + Business Media B.V. 1983

Authors and Affiliations

  • Y. Bashan
    • 1
  • I. Assouline
    • 2
  1. 1.Dept. of Plant Pathology and MicrobiologyThe Hebrew University of Jerusalem, Faculty of AgricultureRehovot
  2. 2.Analyst Ltd.Qiryat Weizmann LeMadahRehovot

Personalised recommendations