, Volume 13, Issue 3–4, pp 173–178 | Cite as

Effect of an aqueous extract of neem seed kernel on larvae of the European corn borer,Ostrinia nubilalis

  • J. Meisner
  • Venezia Melamed-Madjar
  • K. R. S. Ascher
  • S. Tam


An aqueous neem seed kernel extract was sprayed at different concentrations on sweet corn seedlings and assayed against larvae ofOstrinia nubilalis Hb. Larval survival, larval weight and percent pupation served as the criteria of activity. No pupation occurred with fresh residues of 1.0, 0.5 and 0.25% neem extract; only 7% of the larvae pupated at 0.1%, and 16% at 0.05%. The residual effect of the 1% aqueous extract was also investigated, 2, 6 and 8 days after treatment. At all observation times larval weight on the treated leaves was significantly lower than on untreated ones and no pupation occurred even on 8-day-old residues. Systemic properties of neem in the plant were demonstrated by incorporation of the extract into the soil. Quite good control was obtained when the corn seedlings were exposed to larvae 4 and 6 days after the soil treatment, whereas only slight activity was found 2 days after treatment.

Key Words

Ostrinia nubilalis sweet corn aqueous extract of neem (Azadirachta indica) seed kernels neem, residual effect of neem, systemic effect of 


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  1. 1.
    Ascher, K.R.S. (1981) Some physical (solubility) properties and biological (sterilant forEpilachna varivestris females) effects of dried methanolic Neem (Azadirachta indica) seed kernel extract.Proc. 1st Int. Neem Conf. (Rottach-Egern, 1980), pp. 63-74.Google Scholar
  2. 2.
    Butterworth, J.H., Morgan, E.D. and Percy, G.R. (1972) The structure of azadirachtin; the functional groups.J. Chem. Soc. Perkin Trans. I:2445–2450.CrossRefGoogle Scholar
  3. 3.
    Chiu, S.-F. (1984) The active principles and insecticidal properties of some Chinese plants, with special reference to Meliaceae.Proc. 2nd Int. Neem Conf. (Rauischholzhausen, 1983), pp. 255-262.Google Scholar
  4. 4.
    Chiu, S.-F., Zhang, X., Liu, S.-K. and Huang, D.-P. (1984) Growth disrupting effects of azadirachtin on the larvae of the Asiatic corn borer (Ostrinia furnacalis Guenée). Abstr. Vol.XVII Int. Congr. Ent. (Hamburg, 1984), p. 866.Google Scholar
  5. 5.
    Gill, J.S. (1972) Studies on insect feeding deterrents with special reference to the fruit extracts of the Neem tree,Azadirachta indica A. Juss. Ph.D. thesis, University of London, U.K.Google Scholar
  6. 6.
    Gill, J.S. and Lewis, C.T. (1971) Systemic action of an insect feeding deterrent.Nature, Lond. 232:402–403.CrossRefGoogle Scholar
  7. 7.
    Ketkar, S.L.M. (1976) Utilization of Neem (Azadirachta indica A. Juss.) and its by-products. 1st ed. Nana Dengle Sadhana Press, Poona, India.Google Scholar
  8. 8.
    Meisner, J., Ascher, K.R.S. and Aly, R. (1981) The residual effect of some products of Neem seeds on larvae ofSpodoptera littoralis in laboratory and field trials.Proc. 1st Int. Neem Conf. (Rottach-Egern, 1980), pp. 157-170.Google Scholar
  9. 9.
    Melamed-Madjar, V. and Raccah, B. (1979) The transstadial and vertical transmission of a granulosis virus from the corn borerSesamia nonagrioides. J. invert. Path. 33:259–264.CrossRefGoogle Scholar
  10. 10.
    Melamed-Madjar, V. and Tam, S. (1980) A field survey of changes in the composition of corn borer populations in Israel.Phytoparasitica 8:201–204.Google Scholar
  11. 11.
    Nakanishi, K. (1975) Structure of the insect antifeedant azadirachtin.Recent Adv. Phytochem.9:238–298.Google Scholar
  12. 12.
    Pradhan, S. and Jotwani, M.G. (1971) Neem kernel as antifeedant for locust.Sneha-San-desh 13:1–5.Google Scholar
  13. 13.
    Radwanski, S. (1977) Neem tree. 3: Further uses and potential uses.Wld Crops Live Stk 29:167–168.Google Scholar
  14. 14.
    Ruscoe, C.N.E. (1972) Growth disruption effects of an insect antifeedant.Nature, New Biol. 236:159–160.CrossRefGoogle Scholar
  15. 15.
    Skatulla, N. and Meisner, J. (1975) Labor-Versuche mit Neem-Samenextract źur Bekämpfung des Schwammspinners,Lymantria dispar L.Anz. Schädlingsk. PflSchutz Umweltschutz 48:38–40.CrossRefGoogle Scholar
  16. 16.
    Steets, R. (1975) Die Wirkung von Rohextrakten aus den MeliaceenAzadirachta indica undMelia azederach auf verschiedene Insektenarten.Z. angew. Ent. 77:306–312.Google Scholar
  17. 17.
    Warthen, J.D. Jr. (1979)Azadirachta indica: a source of insect feeding inhibitors and growth regulators.U.S. Dep. Agric. ARM-NE-4.Google Scholar

Copyright information

© Springer Science + Business Media B.V. 1985

Authors and Affiliations

  • J. Meisner
    • 1
  • Venezia Melamed-Madjar
    • 2
  • K. R. S. Ascher
    • 1
  • S. Tam
    • 2
  1. 1.Dept. of ToxicologyARO, The Volcani CenterBet DaganIsrael
  2. 2.Dept. of EntomologyARO, The Volcani CenterBet DaganIsrael

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