Abstract
The effect of the root ofCoptis japonica (COPT), both the dichloromethane soluble (CH2Cl2) and insoluble (H2O) fractions, on catecholamine contents and tyrosine hydroxylase (TH) activity in PC12 cells was investigated. CH2Cl2 and H2O fractions showed 21 and 53% inhibitions on dopamine content, respectively, at a concentration of 40 μg/ml in medium: the H2O fraction provided a greater inhibitory effect. The TH activity was reduced by the treatment of COPT (H2O fraction). These results suggest that COPT has an inhibitory effect on the catecholamine biosynthesis by the reduction of TH activity in PC12 cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References Cited
Chen, H. C. and Hsieh, M. T., Two-year experience with “San-Huang-Hsih-Tang” in essential hypertension.Amer. J. Chin. Med., 14, 51–58 (1986).
Franzblau, S. G. and Cross, C., Comparativein vitro antimicrobial activity of chinese medicinal herbs.J. Ethnopharmacol., 15, 279–288 (1986).
Fukushima, M. and Kimura, S., Studies on cosmetic ingredients from crude drugs. I. Inhibition of tyrosinase activity by crude drugs.Shoyakugaku Zasshi, 43, 142–147 (1989).
Greene, L. A. and Rein, G., Short-term regulation of catecholamine biosynthesis in a nerve growth factor responsive clonal line of rat pheochromocytoma cells.J. Neurochem., 30, 549–555 (1977).
Hirai, Y., Takase, H., Kobayashi, H., Yamamoto, M., Fujioka, N., Kohda, H., Yamasaki, K., Yasuhara, T. and Nakajima, T., Screening test for anti-inflammatory crude based on inhibition effect of histamine release from mast cell.Shoyakugaku Zasshi, 37, 374–380 (1983).
Hong, N. D., Koo, B. H., Joo, S. M. and Lee, S. K., Studies on the efficacy of combined praparation of crude drugs (XXXVI), Effects of SIPMIDOJUKSAN on the central nervous and cardiovascular system.Kor. J. Pharmacogn., 19, 141–151 (1988).
Hwang, Y. J., Lee, S. H., Kim, H. S., Lee, K. S., Ro, J. S. and Lee, M. K., Effects of herbal drugs on bovine adrenal tyrosine hydroxylase and dopamine β-hydroxylase (II).Kor. J. Pharmacogn., 25, 194–197 (1994).
Kim, J. H., Lee, S. J., Han, Y. B. and Kim, J. B., Identification of active component isolated fromCroton tiglium andCoptis japonica aqueous mixture (CP2) and studies of its cytotoxic effect.Yakhak Hoeji, 38, 31–37 (1994).
Kumazawa, N., Ohta, S., Tu, S. H., Kamogawa, A. and Shinoda, M., Protective effects of various methanol extracts of crude drugs on experimental hepatic injury induced by α-naphthylisothiocyanate in rats.Yakugaku Zasshi, 111, 199–204 (1991).
Lewis, E. J., Harrington, C. A. and Chikaraishi, D. M., Transcriptional regulation of the tyrosine hydroxylase gene by glucocorticoid and cyclic AMP.Proc. Natl. Acad. Sci. USA, 84, 3550–3554 (1987).
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J., Protein measurement with the Folin phenol reagent.J. Biol. Chem., 193, 265–275 (1951).
Lee, M. K., Nohta, H. and Ohkura, Y., Occurrence of aromatic L-amino acid decarboxylase in human plasma and its assay by high-performance liquid chromatography with fluorescence detection.J. Chromatogr., 378, 329–336 (1986).
Mitsui, A., Nohta, H. and Ohkura, Y., High-performance liquid chromatography of plasma catecholamines using 1,2-diphenylethylenediamine as precolumn fluorescence derivatization reagent.J. Chromatogr., 344, 61–70 (1985).
Nagatsu, T., Oka, K. and Kato, K., Highly sensitive assay for tyrosine hydroxylase activity by high-performance liquid chromatography.J. Chromatogr., 163, 247–252, (1979).
Riberio, P., Pigeon, D. and Kaufman, S., The hydroxylation of phenylalanine and tyrosine by tyrosine hydroxylase from cultured pheochromocytome cells.J. Biol. Chem., 266, 16207–16211 (1991).
Stachowiak, M. K., Rigual, R. J., Lee, P. H. K., Viveros, O. H. and Hong, J. S., Regulation of tyrosine hydroxylase and phenolethanolamine N-methyltransferase mRNA levels in the sympathoadrenal system by the pituitary-adrenocortical axis.Mol. Brain Res., 3, 275–286 (1988).
Takase, H., Imanishi, K., Miura, O. and Yumioka, E., A possible mechanism for the gastric mucosal protection by OREN-GEDOKU-TO (OGT), A traditional herbal medicine.Jpn. J. Pharmacol., 51, 17–23 (1987).
Tischler, A. S., Perlman, R. L., Morse, G. M. and Sheard, B. E., Glucocorticoids increase catecholamine synthesis and storage in PC12 pheochromocytoma cell cultures.J. Neurochem., 40, 364–370 (1983).
Yamahara, J., Behavioral pharmacology of berberine-type alkaloids(1), Central depressive action of Coptidis Rhizoma and its constituents.Nippon Yakurigaku Zasshi, 72, 899–908 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lee, M.K., Park, W.K. & Kim, H.S. Inhibitory effect of the root ofCoptis japonica on catecholamine biosynthesis in PC12 cells. Arch. Pharm. Res. 17, 269–272 (1994). https://doi.org/10.1007/BF02980459
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02980459