Archives of Pharmacal Research

, Volume 17, Issue 4, pp 269–272 | Cite as

Inhibitory effect of the root ofCoptis japonica on catecholamine biosynthesis in PC12 cells

  • Myung Koo Lee
  • Woo Kyu Park
  • Hack Seang Kim
Research Articles


The effect of the root ofCoptis japonica (COPT), both the dichloromethane soluble (CH2Cl2) and insoluble (H2O) fractions, on catecholamine contents and tyrosine hydroxylase (TH) activity in PC12 cells was investigated. CH2Cl2 and H2O fractions showed 21 and 53% inhibitions on dopamine content, respectively, at a concentration of 40 μg/ml in medium: the H2O fraction provided a greater inhibitory effect. The TH activity was reduced by the treatment of COPT (H2O fraction). These results suggest that COPT has an inhibitory effect on the catecholamine biosynthesis by the reduction of TH activity in PC12 cells.

Key words

Coptis japonica Catecholamines Tyrosine Hydroxylase PC12 cells 


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Copyright information

© The Pharmaceutical Society of Korea 1994

Authors and Affiliations

  • Myung Koo Lee
    • 1
  • Woo Kyu Park
    • 1
  • Hack Seang Kim
    • 1
  1. 1.College of PharmacyChungbuk National UniversityCheongjuKorea

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