Abstract
Enzyme-amplified ELISA (enzyme-linked immunosorbent assay) increased the sensitivity of detection of citrus tristeza virus and papaya ringspot virus in plant sap by 25- and 125-fold, respectively, compared with direct double antibody sandwich ELISA. The advantages of the new substrate system for the detection of low concentrations of viruses in plants are discussed.
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Ben-Zeev, I.S., Frank, A. & Bar-Joseph, M. Sensitive detection of two plant viruses by enzyme-amplified elisa. Phytoparasitica 16, 343–349 (1988). https://doi.org/10.1007/BF02979510
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DOI: https://doi.org/10.1007/BF02979510