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Phytoparasitica

, Volume 16, Issue 4, pp 343–349 | Cite as

Sensitive detection of two plant viruses by enzyme-amplified elisa

  • I. S. Ben-Zeev
  • A. Frank
  • M. Bar-Joseph
Article

Abstract

Enzyme-amplified ELISA (enzyme-linked immunosorbent assay) increased the sensitivity of detection of citrus tristeza virus and papaya ringspot virus in plant sap by 25- and 125-fold, respectively, compared with direct double antibody sandwich ELISA. The advantages of the new substrate system for the detection of low concentrations of viruses in plants are discussed.

Key Words

Enzyme-amplified ELISA Papaya ringspot virus (PRSV) Citrus tristeza virus (CTV) 

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Copyright information

© Springer Science + Business Media B.V. 1988

Authors and Affiliations

  • I. S. Ben-Zeev
  • A. Frank
  • M. Bar-Joseph
    • 1
  1. 1.Dept. of VirologyARO, The Volcani CenterBet DaganIsrael

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