Sensitive detection of two plant viruses by enzyme-amplified elisa
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Enzyme-amplified ELISA (enzyme-linked immunosorbent assay) increased the sensitivity of detection of citrus tristeza virus and papaya ringspot virus in plant sap by 25- and 125-fold, respectively, compared with direct double antibody sandwich ELISA. The advantages of the new substrate system for the detection of low concentrations of viruses in plants are discussed.
Key WordsEnzyme-amplified ELISA Papaya ringspot virus (PRSV) Citrus tristeza virus (CTV)
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