Comparison of spectrophotometric, HPLC and chemilumines-cence methods for 3-nitrotyrosine and peroxynitrite interaction

  • Nilufer Nermin Turan
  • Mustafa Ark
  • Abdullah Tuncay Demiryurek
Drug efficacy


We have studied the interaction of 3-nitrotyrosine with peroxynitrite using three different methods; chemiluminescence, spectrophotometry and HPLC. Peroxynitrite-induced luminol or lucigenin chemiluminescence were significantly decreased by 3-nitrotyrosine, in concentration-dependent manners. The intensity of the peroxynitrite spectrum was also markedly reduced in the presence of 3-nitrotyrosine in the spectrophometric assay. However, there was no attenuation of the 3-nitrotyrosine signal in the HPLC assay after mixing with peroxynitrite. The interaction of 3-nitrotyrosine and hypochlorous acid (HOCI) was also studiedvia the chemiluminescence assay, where the HOCI-induced responses were markedly inhibited by 3-nitrotyrosine. These results suggest that caution should be taken when studying the levels or interactions of 3-nitrotyrosine.

Key words

3-Nitrotyrosine Peroxynitrite Spectrophometric assay Chemiluminescence assay 


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Copyright information

© The Pharmaceutical Society of Korea 2005

Authors and Affiliations

  • Nilufer Nermin Turan
    • 3
  • Mustafa Ark
    • 1
    • 3
  • Abdullah Tuncay Demiryurek
    • 2
    • 3
  1. 1.Faculty of Medicine, Department of PharmacologyUniversity of MersinMersinTurkey
  2. 2.Faculty of Medicine, Department of PharmacologyUniversity of GaziantepGaziantepTurkey
  3. 3.Faculty of Pharmacy, Department of PharmacologyGazi UniversityEtiler, AnkaraTurkey

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