Abstract
We have studied the interaction of 3-nitrotyrosine with peroxynitrite using three different methods; chemiluminescence, spectrophotometry and HPLC. Peroxynitrite-induced luminol or lucigenin chemiluminescence were significantly decreased by 3-nitrotyrosine, in concentration-dependent manners. The intensity of the peroxynitrite spectrum was also markedly reduced in the presence of 3-nitrotyrosine in the spectrophometric assay. However, there was no attenuation of the 3-nitrotyrosine signal in the HPLC assay after mixing with peroxynitrite. The interaction of 3-nitrotyrosine and hypochlorous acid (HOCI) was also studiedvia the chemiluminescence assay, where the HOCI-induced responses were markedly inhibited by 3-nitrotyrosine. These results suggest that caution should be taken when studying the levels or interactions of 3-nitrotyrosine.
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Turan, N.N., Ark, M. & Demiryurek, A.T. Comparison of spectrophotometric, HPLC and chemilumines-cence methods for 3-nitrotyrosine and peroxynitrite interaction. Arch Pharm Res 28, 358–363 (2005). https://doi.org/10.1007/BF02977805
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DOI: https://doi.org/10.1007/BF02977805