Archives of Pharmacal Research

, Volume 28, Issue 5, pp 529–533 | Cite as

Free Radical Scavenging and Hepatoprotective Constituents from the Leaves of Juglans sinensis

  • Ren-Bo An
  • Hyun-Chul Kim
  • Yu-Hua Tian
  • Youn-Chul Kim


In the course of searching for hepatoprotective agents from natural products, six compounds were isolated from the MeOH extract of the leaves of Juglans sinensis, as guided by their DPPH free radical scavenging activity. The structures were determined as juglanoside B (1), quercetin 3-O-α-L-arabinofuranoside (avicularin, 2), quercetin 3-O-α-L-arabinopyranoside (guaijaverin, 3), quercetin 3-O-α-L-rhamnopyranoside (quercitrin, 4), (+)-catechin (5) and quercetin 3-O-β-D-galactopyranoside (hyperin, 6). Compounds 2-6 showed significant DPPH free radical scavenging effects. An evaluation for the hepatoprotective activity of the isolated compounds on drug-induced cytotoxicity was conducted, and compounds 1, 2, and 5 showed protective effects against nitrofurantoin-induced cytotoxicity, and compound 5 also exhibited a moderate protective effect on amiodarone-induced cytotoxicity in Hep G2 cells.

Key words

Juglans sinensis Juglandaceae Hepatoprotective Hep G2 Cells Free radical scavenging 


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Copyright information

© The Pharmaceutical Society of Korea 2005

Authors and Affiliations

  • Ren-Bo An
    • 1
    • 2
  • Hyun-Chul Kim
    • 1
  • Yu-Hua Tian
    • 1
  • Youn-Chul Kim
    • 1
  1. 1.College of PharmacyWonkwang UniversityIksanKorea
  2. 2.College of PharmacyYanbian UniversityYanjiChina

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