Resveratrol-induced depression of the mechanical and electrical activities of the rat heart is reversed by glyburide: evidence for possible KATP channels activation
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Resveratrol, a natural phytoalexin found in wine, has been suggested to have benefits in preventing cardiovascular diseases. However, the direct effects of resveratrol on the activity of cardiac tissues and its mechanism of action have not been determined. This study examined the effects of resveratrol on the right and left atrium and left papillary muscle isolated from the rat heart. The contractile responses of the right atrium and papillary muscle and the action potential from the left atrium were recorded and the effects of resveratrol on these responses were observed. The resting force of the isolated right atrium and the peak developed force of the left papillary muscle were depressed by resveratrol (0.1 nM – 0.1 mM). Exposure to the KATP channel blocker glyburide (3 μM) prevented significantly the resveratrol-induced decrease. Resveratrol (0.1 mM) shortened the repolarization phase of action potential recorded from the left atrium and this effect of resveratrol was reversed by glyburide (3 μM). These results indicated that resveratrol depressed cardiac muscle contraction and shortened action potential duration probably due to the activation of KATP channels in the rat heart.
Key wordsResveratrol Rat heart Contraction Membrane potential KATP channels
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- Buluc, M. and Demirel-Yilmaz, E., Possible mechanism for depression of smooth muscle tone by resveratrol. In: Adv. Rec. Cardiovas. Res. Ed.: A. Varro, A. Vegh. Monduzzi Editore, Bologna, Italy, pp. 55–59, (2002).Google Scholar
- Buluc, M. and Demirel-Yilmaz, E., Resveratrol decreases calcium sensitivity of vascular smooth muscle and enhances cytosolic calcium increase in endothelium.Vas. Pharmacol., 44(4), 231–237(2006).Google Scholar
- Chen, C. C., Lin, Y. C., Chen, S. A., Luk, H. N., Ding, P. Y. A., Chang, M. S., and Chiang, C. E., Shortening of cardiac action potentials in endotoxic shock in guinea pigs is caused by an increase in nitric oxide activity and activation of the adenosine triphosphate-sensitive potassium channel.Crit. Care Med., 28, 1713–1720 (2000).PubMedCrossRefGoogle Scholar
- Han, J., Kim, N., Joo, H., Kim, E., and Earm, Y. E., ATP- sensitive K+ channels activation by nitric oxide and protein kinase G in rabbit ventricular myocytes.Am. J. Physiol., 283, H1545-H1554 (2002).Google Scholar
- Jager, U. and Nguyen-Duong, H., Relaxant effect of trans-resveratrol on isolated porcine coronary arteries.Arzneim. Forsch., 49, 207–211 (1999).Google Scholar
- Wahler, G. M. and Sperelakis, N., Intracellular injection of cyclic GMP depresses cardiac slow action potentials.J. Cyclic Nucleotid Protein Phosphor. Res., 10, 83–95 (1985).Google Scholar
- Zhao, J., Ma, H. J., Dong, J. H., Zhang, L. P., Liu, H. L., and Wang, Q. S., Electrophysilogical effects of resveratrol on guinea pig papillary muscles.Acta Physiol. Sin., 56, 708–712 2004).Google Scholar