Hepatoprotective activity of scopoletin, a constituent ofSolanum lyratum

  • So Young Kang
  • Sang Hyun Sung
  • Jong Hee Park
  • Young Choong Kim
Research Articles


Scopoletin (7-hydroxy-6-methoxycoumarin), a coumarin, was isolated from the aerial part ofSolanum lyratum Thunb. by the activity-guided fractionation employing carbon tetrachloride-intoxicated primary cultured rat hepatocytes as a screening system. Its hepatoprotective activity was first evaluated by measuring the release of glutamic pyruvic transaminase and sorbitol dehydrogenase from carbon tetrachloride-intoxicated rat hepatocytes into the culture medium. Scopoletin significantly reduced the releases of glutamic pyruvic transaminase and sorbitol dehydrogenase from the carbon tetrachloride-intoxicated primary cultured rat hepatocytes by 53% and 58%, respectively, from the toxicity in a dose-dependent manner over concentration ranges of 1 μM to 50 μM. Further studies revealed that at the concentration of 10 μM, scopoletin significantly preserved glutathione content by 50% and the activity of superoxide dismutase by 36% and also inhibited the production of malondialdehyde to the degree as seen in the control.

Key words

Hepatoprotective activity Scopoletin Solanum lyratum Carbon tetrachloride Primary cultured rat hepatocytes 

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Copyright information

© The Pharmaceutical Society of Korea 1998

Authors and Affiliations

  • So Young Kang
    • 1
  • Sang Hyun Sung
    • 1
  • Jong Hee Park
    • 2
  • Young Choong Kim
    • 1
  1. 1.College of PharmacySeoul National UniversitySeoulKorea
  2. 2.College of PharmacyPusan National UniversityPusanKorea

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