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Archives of Pharmacal Research

, Volume 23, Issue 5, pp 518–524 | Cite as

Differential expression of protein kinase C subtypes during ginsenoside Rh2-induced apoptosis in SK-N-BE(2) and C6Bu-1 cells

  • Young Sook Kim
  • Sung Ha Jin
  • You Hiu Lee
  • Jong Dae Park
  • Shin Il Kim
Research Articles Biomedical Sciences

Abstract

We examined the modulation of protein kinase C (PKC) subtypes during apoptosis induced by ginsenoside Rh2 (G-Rh2) in human neuroblastoma SK-N-BE(2) and rat glioma C6Bu-1 cells. Apoptosis induced by G-Rh2 in both cell lines was confirmed, as indicated by DNA fragmentation andin situ strand breaks, and characteristic morphological changes. During apoptosis induced by G-Rh2 in SK-N-BE(2) cells, PKC subtypes α, β and γ were progressively increased with prolonged treatment, whereas PKC δ increased transiently at 3 and 6 h and PKC ε was gradually down-regulated after 6 h following the treatment. On the other hand, PKC subtype ζ markedly increased at 24 h when maximal apoptosis was achieved. In C6Bu-1 cells, no significant changes in PKC subtypes α, γ, δ, ε and ζ were observed during apoptosis induced by G-Rh2. These results suggest the evidence for a possible role of PKC subtype in apoptosis induced by G-Rh2 in SK-N-BE(2) cells but not in C6Bu-1 cells, and raise the possibility that G-Rh2 may induce apoptosis via different pathways interacting with or without PKC in different cell types.

Key words

Ginsenoside Rh2 Apoptosis Protein kinase C SK-N-BE(2) cells C6Bu-1 cells 

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Copyright information

© The Pharmaceutical Society of Korea 1999

Authors and Affiliations

  • Young Sook Kim
    • 1
  • Sung Ha Jin
    • 1
  • You Hiu Lee
    • 1
  • Jong Dae Park
    • 1
  • Shin Il Kim
    • 1
  1. 1.Korea Ginseng & Tobacco Research InstituteTaejonKorea

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