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Archives of Pharmacal Research

, Volume 22, Issue 2, pp 108–112 | Cite as

Inhibition of stem cell factor- and nerve growth factor-induced morphological change by wortmannin in mast cells

  • Hyung-Min Kim
  • Young-Hoe Moon
  • Nyun-Hyung An
Research Articles Biochemistry & Cell Biology

Abstract

Recombinant murine stem cell factor (rmSCF) or recombinant murine nerve growth factor (rmNGF) induced the morphological change of large numbers of rat peritoneal mast cells (RPMC). We investigated the role of phosphatidylinositol 3′-kinase (PI3-kinase) in receptors-mediated morphological change in RPMC. Exposure of RPMC to PI3-kinase inhibitor, wortmannin, before the addition of rmSCF and rmNGF antagonized those factors-induced morphological change. These results suggest that the PI3-kinase is involved in the signal transduction pathway responsible for morphological change following stimulation of rmSCF and rmNGF and that wortmannin blocks these responses.

Key words

Phosphatidylinositol 3′-kinase Stem cell factor Nerve growth factor Morphological change Rat peritoneal mast cells 

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Copyright information

© The Pharmaceutical Society of Korea 1999

Authors and Affiliations

  • Hyung-Min Kim
    • 1
  • Young-Hoe Moon
    • 2
  • Nyun-Hyung An
    • 1
  1. 1.College of PharmacyWonkwang UniversityIksan, ChonbukKorea
  2. 2.School of MedicineWonkwang UniversityIksan, ChonbukKorea

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