Electrochemical behavior and differential pulse polarographic determination of rifampicin in the pharmaceutical preparations

Research Articles Medicinal Chemistry & Natural Products


Differential pulse polarographic (DPP) analytical procedure for the rifampicin antibiotic, which can be applied to monitor its synthetic process from the starting antibiotic of rifamycin B or rifamycin SV, has been developed based on the electrochemical reduction of an azomethine group. Rifampicin exhibited a cathodic peak due to the azomethine group in the side chain of 3-[(4-methyl-1-piperazinyl)imino]methyl moiety and another cathodic peak due to the carbonyl group in rifamycin SV by DPP. The experimental peak potential shift of an azomethine reduction was −73 mV/pH in the pH range between 3.0 and 7.5, agreeing with involvement of 4 e and 5 H+ in its reduction. By the cyclic voltammetric(CV) studies, the azomethine and the carbonyl reductions in rifampicin were processed irreversibly on the mercury electrode. The plot of peak currents vs. concentrations of rifampicin ranging 1.0×10−7 M∼1.0×10−5 M yielded a straight line with a correlation coefficient of 0.9996. The detection limit was 1.0×10−8 M with a modulation amplitude of 50 mV. DPP has been successfully applied for the determination of rifampicin in the pharmaceutical preparations.

Key words

Differential pulse polarography Cyclic voltammetry Rifampicin 


  1. Argekar, A. P., Kunjir, S. S. and Purandare, K. S., Simultaneous determination of rifampicin, isoniazid and pyrazinamid by high performance thin layer chromatography,J. Pharm. Biomed. Anal., 14, 1645–1650 (1996).PubMedCrossRefGoogle Scholar
  2. Blaedel, W. J. and Hahn, Y., Investigation of diazepam by pulsed rotation voltammetry,Arch. Pharm. Res., 2(2), 111–114 (1979).CrossRefGoogle Scholar
  3. British Pharmacopoeia Commission,British Pharmacopoeia, The Stationery Office, London, 1346–1347, 2000.Google Scholar
  4. European Pharmacopoeia Commission,European Pharmacopeia, Council of Europe, Strasbourg, 1446–1447 (1997).Google Scholar
  5. Galal, S. M., Blaih, S. M., and Abdel-Hamid, M. E., Comparative spectrophotometric analysis of rifampicin by chelate formation and charge-transfer complexation,Anal. Lett., 25(4), 725–743 (1992).Google Scholar
  6. Gallo, G. G., Chiesa, L., and Sensi, P., Rifamycin XXIII. The polarographic behavior of rifamycin B, rifamycin O, rifamycin S, and rifamycin SV,Anal. Chem., 34(3), 423–426 (1962).CrossRefGoogle Scholar
  7. Hahn, Y. and Son, E., Electrochemical behavior and differential pulse polarographic determination of piperacillin sodium,Arch. Pharm. Res., 23(2), 197–201 (2000).PubMedCrossRefGoogle Scholar
  8. Jindal, K. C., Chaudhary, R. S., Gangwal, S. S., Singla, A. K., and Khanna, S., High-performance thin-layer chromatographic method for monitoring degradation products of rifampicin in drug excipient interaction studies,J. Chromato. A, 685, 195–199 (1994).CrossRefGoogle Scholar
  9. Korea Food and Drug Adminstration,The minimum Requirements for Antibiotic Products of Korea, Yakup Shinmoon Co., Korea, 55–58 (2000).Google Scholar
  10. Lund, H., Reduction of azomethine compounds, In Lund, H. and Baizer, M. M. (Eds.)Organic electrochemistry, an introduction and a guide, 3rd ed. Marcel Dekker, New York, 465–466 (1991).Google Scholar
  11. United States Pharmacopeial Convention, U. S. Pharmacopeia & National Formulary, National Publishing, Philadelphia, PA, 1484–1487 (2000).Google Scholar
  12. Walash, M. I., Belal, F., Metwally, M. E., and Hefnawy, M. Mt., Spectrophotometric determination of rifampicin in the presence of its degradation products in pharmaceutical preparations,Anal. Lett., 26(9), 1905–1917 (1993).Google Scholar

Copyright information

© The Pharmaceutical Society of Korea 2001

Authors and Affiliations

  1. 1.Department of ChemistrySangmyung UniversitySeoulKorea

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