Comparison between of the attenuated BR-Oka and the wild type strain of Varicella Zoster Virus (VZV) on the DNA level
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Oka strain VR-795 (Varicella Zoster Virus, VZV) of American Type Culture Collection (ATCC) has been used for chickenpox vaccine production. In order to use this strain for vaccine production, the strain must be identified and its stability must be confirmed. The identification of the Oka strain has been confirmed using Restriction Fragment Length Polymorphism (RFLP) and DNA sequence analysis of glycoprotein-II (gp-II). The amino acid sequences of Oka deduced from the DNA sequence of gp-II have changed at three amino acids against Ellen and at one amino acid against Webster. To prove the stability of the Oka strain during the passage, RFLP and DNA sequence analyses were also used with 11, 15 and 23 times of virus passage. We found that the Oka strain was stable at passages of up to 23 times, based on the RFLP and DNA sequence analyses. The confirmed Oka strain was renamed as BR-Oka for the purposes of chickenpox vaccine production.
Key wordsVZV Oka Chicken pox Vaccine
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- Hardy, I. and Gershon, A. A., Prospects for use of a varicella vaccine in adults.Infect. Dis. Clin. North Am., 4, 160–173 (1990).Google Scholar
- Sambrook, J., Fritisch, E. F. and Maniatis, T.,Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989.Google Scholar
- Yoshizo, A., Takao, N., Takao, M., Takehiko, Y., Shigemitsu, I., Koichi, Y. and Michiaki, T., Long term protective immunity of recipients of the Oka strain of live varicella vaccine.PEDIATRICS, 75(4), 667–671 (1985).Google Scholar
- Weller, T. H., Serial propagation in vitro of agents producing inclusion bodies derived from varicella and herpes zoster.Proc. Soc. Exp. Bio. Med. 83, 340–346 (1953).Google Scholar
- Wolfgang, K. J.,Virology, 3rd ED., Prentice-Hall, Englewood Cliffs, New Jersey, (1988).Google Scholar