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Archives of Pharmacal Research

, Volume 23, Issue 4, pp 407–412 | Cite as

Identification and characterization of nitric oxide synthase inSalmonella typhimurium

  • Don Woong Choi
  • Hye Young Oh
  • Sung Youl Hong
  • Jeung Whan Han
  • Hyang Woo Lee
Research Articles Biomedical Sciences

Abstract

The presence of the nitric oxide synthase (NOS) enzyme fromSalmonella typhimurium (S. typhimurium) was identified by measuring radiolabeled L-[3H]citrulline and NO, and Western blot analysis. NOS was partially purified by both Mono Q ion exchange and Superose 12HR size exclusion column chromatography, sequentially. The molecular weight of NOS was estimated to be 93.3 kDa by Western blot analysis. The enzyme showed a significant dependency on the typical NOS cofactors; an apparent Km for L-arginine of 34.7 mM and maximum activity between 37°C and 43°C. The activity was inhibited by NOS inhibitors such as aminoguanidine and NG,NG-dimethyl-L-arginine. Taken together, partially purified NOS inS. typhimurium is assumed to be a different isoform of mammalian NOSs.

Key words

Salmonella typhimurium Nitric oxide Nitric oxide synthase 

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Copyright information

© The Pharmaceutical Society of Korea 1999

Authors and Affiliations

  • Don Woong Choi
    • 1
  • Hye Young Oh
    • 2
  • Sung Youl Hong
    • 3
  • Jeung Whan Han
    • 1
  • Hyang Woo Lee
    • 1
  1. 1.College of PharmacySungkyunkwan UniversitySuwon cityKorea
  2. 2.Department of Toxicology, National Institute of Toxicological ResearchKFDASeoulKorea
  3. 3.Department of Genetic Engineering, College of Life Science and Natural ResourcesSungkyunkwan UniversitySuwon cityKorea

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