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Archives of Pharmacal Research

, Volume 23, Issue 4, pp 338–343 | Cite as

Activity of crude extract ofRubus crataegifolius roots as a potent apoptosis inducer and DNA topoisomerase I inhibitor

  • Ji Hyeon Lee
  • Yoon-Ah Ham
  • Sang-Ho Choi
  • Eun Ok Im
  • Jee H. Jung
  • Kwang Sik Im
  • Dong-Kyoo Kim
  • Ying Xu
  • Min Wei Wang
  • Nam Deuk Kim
Research Articles Medicinal Chemistry & Natural Products

Abstract

The effects of methanol extract ofRubus crategifolius roots and its solvent fractions were investigated on the proliferation of MCF-7 human breast carcinoma cells. The methanol extract inhibited the proliferation of MCF-7 cells in a concentration dependent manner. Moreover, their methanol soluble (W-M) fraction had the greatest inhibitory effect on the growth of MCF-7 cells. To evaluate whether the W-M fraction affects on the cell cycle of MCF-7 cells, cells treated with this fraction were analyzed with flow cytometry. The W-M fraction increased G0/G1 phase after 24 h-treatment and induced apoptosis after 48 h-treatment. The hallmark of apoptosis, DNA fragmentation, also appeared by W-M fraction after 48 h-treatment. Furthermore the methanol extract and its W-M fraction inhibited the activity of the topoisomerase I enzyme in the relaxation assay. From these results, their W-M fraction as well as methanol extract ofR. crategifolius roots are necessary for further studies as a potent inhibitor of the growth of cancer cells.

Key words

Rubus crategifolius roots MCF-7 cells Topoisomerase I inhibitor Cell cycle Apoptosis DNA fragmentation 

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Copyright information

© The Pharmaceutical Society of Korea 1999

Authors and Affiliations

  • Ji Hyeon Lee
    • 1
  • Yoon-Ah Ham
    • 1
  • Sang-Ho Choi
    • 1
  • Eun Ok Im
    • 1
  • Jee H. Jung
    • 1
  • Kwang Sik Im
    • 1
  • Dong-Kyoo Kim
    • 2
  • Ying Xu
    • 3
  • Min Wei Wang
    • 3
  • Nam Deuk Kim
    • 1
  1. 1.College of PharmacyPusan National UniversityPusanKorea
  2. 2.Dept. of ChemistryInje UniversityKimhaeKorea
  3. 3.Pharmacological Teaching Group, Dept. of PharmacyShenyang Pharmaceutical UniversityShenyangChina

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