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Degradation of acharan sulfate and heparin byBacteroides stercoris HJ-15, a human intestinal bacterium

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Abstract

When glycosaminoglycan (GAG)-degrading enzymes were measured in normal human stool suspensions, all 5 tested different stools degraded titrable heparin and acharan sulfate. GAG-degrading bacteria were screened from the isolates of human stools. Among them, HJ-15 had the most potent activities of heparinases (GAGs-degrading enzymes). However, HJ-15 produced the enzyme even if in the media without heparin. Acharan sulfate lyase was induced by acharan sulfate and heparin. Heparinase production was also induced by these GAGs. These enzymes, acharan sulfate lyase and heparinase, were produced in exponential and stationary phase of HJ-15 growth, respectively. Optimal pHs of the acharan sulfate lyase and heparinase activities were 7.2 and 7.5, respectively. The biochemical properties of HJ-15 was similar to those ofB. stercoris. However, difference fromB. stercoris was utilization of raffinose. This HJ-15 also degraded chondroitin sulfates A and C.

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Authors and Affiliations

  1. College of Pharmacy, Kyung Hee University, #1 Hoegi, Dongdaemun-ku, 130-701, Seoul, Korea

    Dong-Hyun Kim, Byung-Taek Kim & Sun-Yong Park

  2. Department of Food and Nutrition, Kyung Hee University, #1 Hoegi, Dongdaemun-ku, 130-701, Seoul, Korea

    Na-Young Kim & Myung Joo Han

  3. Natural Products Research Institute, Seoul National University, #28, Yonkundong Jongro-ku, 110-460, Seoul, Korea

    Kuk-Hyun Shin, Wan-Suk Kim & Yeong-Sik Kim

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  1. Dong-Hyun Kim
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  2. Byung-Taek Kim
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  4. Na-Young Kim
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Kim, DH., Kim, BT., Park, SY. et al. Degradation of acharan sulfate and heparin byBacteroides stercoris HJ-15, a human intestinal bacterium. Arch. Pharm. Res. 21, 576–580 (1998). https://doi.org/10.1007/BF02975378

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  • DOI: https://doi.org/10.1007/BF02975378

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