Distribution of bacteria with the arylsulfate sulfotransferase activity
- 74 Downloads
This study is to predict the possible roles of the arylsulfate sulfotransferase (ASST) in the microorganism. At first we studied the spectrum of a distribution of the ASST enzyme through about 1,300 bacteria and the several selected strains were compared withKlebsiella K-36 previously reported in the level of DNA homology using the Southern blot method. From this study, we could predict that this enzyme would not exist in specific bacteria and it might not be a critical enzyme for the life of bacteria.
Key wordsArylsulfate sulfotransferase Screening 4-Methylumbelliferyl sulfate Southern blotting pKASST100
Unable to display preview. Download preview PDF.
- Koizume, M., Shimizu, M., and Kobashi, K., Enzymatic sulfation of quercetin by arylsulfotransferase from a human intestinal bacterium.Chem. Pharm. Bull. 38, 794–796 (1990).Google Scholar
- Sambrook, J., Fritsch, E. F. and Maniatis, T.,Molecular Cloning. A laboratory manual, 2nd ed, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1989.Google Scholar