Abstract
This study is to predict the possible roles of the arylsulfate sulfotransferase (ASST) in the microorganism. At first we studied the spectrum of a distribution of the ASST enzyme through about 1,300 bacteria and the several selected strains were compared withKlebsiella K-36 previously reported in the level of DNA homology using the Southern blot method. From this study, we could predict that this enzyme would not exist in specific bacteria and it might not be a critical enzyme for the life of bacteria.
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References Cited
Baek, M. C., Kim, S. K., Kim, D. H., Kim, B. K. and Choi, E. C., Cloning and sequencing of theKlebsiella K-36astA gene, encoding an arylsulfate sulfotransferase.Microbiol. Immunol. 40, 531–537 (1996).
Baek, M. C., Choi, K. H., Oh, T. G., Kim, D. H., and Choi, E. C., Overexpression of arylsulfate sulfotransferase as fusion protein with glutathione S-transferase.Protein Expr. Purif. 11, 257–262 (1997).
Jaurin, B. and Grindstrom, T.,ampC cephalosporinase ofEscherichia coli K-12 has a different evolutionary origin from that of β-lactamases of the penicillinase type.Proc. Natl. Acad. Sci. USA 78, 1199–1203 (1981).
Kim, D. H., Konishi, L. and Kobashi, K., Purification, characterization and reaction mechanism of novel arylsulfotransferase obtained from an anaerobic bacterium of human intestine.Biochim. Biophys. Acta. 872, 33–41 (1986).
Kim, D. H. and Kobashi, K., Kinetic studies on a novel sulfotransferase fromEubacterium A-44, a human intestinal bacterium.,J. Biochem., 109, 45–48 (1991).
Kim, D. H., Hyun, S. H., Shim, S. B. and Kobashi, K., The role of intestinal bacteria in the transformation of sodium picosulfate.Japan. J. Pharmacol. 59, 1–5 (1992).
Kim, D. H., Kim, H. S. and Kobashi, K., Purification and characterization of novel sulfotransferase obtained fromKlebsiella K-36, an intestinal bacterium of rat.J. Biochem. 112, 456–460 (1992).
Kim, D. H., Yoon, H. K., Koizumi, M., and Kobashi, K., Sulfation of phenolic antibiotics by sulfotransferase obtained from a human intestinal bacterium.Chem. Pharm. Bull. 40, 1056–1057 (1992).
Kim, D. H., Kim, H. S., Imamura, L. and Kobashi, K., Kinetic studies on a sulfotransferase fromKlebsiella K-36, a rat intestinal bacterium.Biol. Pharm. Bull., 17, 543–545 (1994).
Kim, D. H., Kim, B., Kim, H. S., Sohng, I. S., and Kobashi, K., Sulfation of parabens and tyrosylpeptides by bacterial arylsulfate sulfotransferase.Biol. Pharm. Bull. 17, 1326–1328 (1994).
Kobashi, K., and Kim, D. H., A novel sulfotransferase sulfates tyrosine-containing peptides and proteins.Biochem. Biophys. Res. Commun. 140, 38–42 (1986).
Koizume, M., Shimizu, M., and Kobashi, K., Enzymatic sulfation of quercetin by arylsulfotransferase from a human intestinal bacterium.Chem. Pharm. Bull. 38, 794–796 (1990).
Lee, N. S., Kim, B. T., Kim, D. H., and Kobashi, K., Purification and reaction mechanism of arylsulfate sulfotransferase fromHaemophilus K-12, a mouse intestinal bacterium.J. Biochem. 118, 796–801 (1995).
Muramatsu, R., Nukui, E., Sukesada, A., Misawa, S., Komatsu, Y., Okayama, T., Wada, K., Morikawa, T., Hayashi, H., and Kobashi, K., Enzymic O-sulfation of tyrosine residues in hirudins by sulfotransferase fromEubacterium A-44.Eur. J. Biochem. 223, 243–248 (1994).
Sambrook, J., Fritsch, E. F. and Maniatis, T.,Molecular Cloning. A laboratory manual, 2nd ed, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1989.
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Baek, MC., Kwon, AR., Chung, YJ. et al. Distribution of bacteria with the arylsulfate sulfotransferase activity. Arch. Pharm. Res. 21, 475–477 (1998). https://doi.org/10.1007/BF02974646
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DOI: https://doi.org/10.1007/BF02974646