Effects of barbiturates on the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene in native and model membranes
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Synaptosomal plasma membrane vesicles (SPMV) were isolated from fresh bovine cerebral cortex. The effects of barbiturates on the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene (DPH) in intact SPMV and model membranes of total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from SPMV were examined. Barbiturates decreased the rotational relaxation time of DPH in intact SPMV in a dose-dependent manner. In contrast, they did not affect the rotational relaxation time of DPH in SPMVTL and even dose-dependently increased the rotational relaxation time of DPH in SPMVPL.
KeywordsBarbiturates native and model membranes fluidity fluorescent probe technique
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