Archives of Pharmacal Research

, Volume 21, Issue 2, pp 135–139 | Cite as

Production of antibody against saikosaponin a, an active component of bupleuri radix

  • Da-Woon Jung
  • Chung Ki Sung
Research Articles


High titer rabbit polyclonal antibodies (pAbs) which show a specificity for saikosaponin a (SSA), have been generated. The immunogen used was a conjugate of SSA linked through its glucose moiety to bovine serum albumin by periodate oxidation method. The antibody titers obtained from two rabbits, innoculated with the immunogen, reached a plateau after the fourth and third booster injection, respectively. The specificity of the pAbs was determined by hapten inhibition assays using several SSA-like structures. SSA competitively inhibited the binding of the rabbit anti-SSA pAbs to SSA-ovalbumin on solid phase, a coated antigen on the well. The antibodies showed high specificity to SSA, exhibiting no significant cross-reactivity with any of SSA analogues tested.

Key words

Saikosaponin a Polyclonal antibodies Periodate oxidation Hapten inhibition assay 


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Copyright information

© The Pharmaceutical Society of Korea 1998

Authors and Affiliations

  • Da-Woon Jung
    • 1
  • Chung Ki Sung
    • 1
  1. 1.College of PharmacyChonnam National UniversityKwangjuKorea

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