Advertisement

In vitro stimulation of murine peritoneal monocytes induced by alginates

  • Paolo Pasquali
  • Amy Zalcman
  • Susanna Murtas
  • Rosanna Adone
  • Gianfranco Brambilla
  • Cinzia Marianelli
  • Monica Cagiola
  • Franco Ciuchini
Articles Drug Development

Abstract

In this trial we assessed the effect of soluble alginates on murine cells. Mouse peritoneal monocytes were stimulatedin vitro with a solution of alginate. The production of TNF-α and nitric oxide (NO), the expression of surface molecules CD80 and CD86, and the ability of monocytes to phagocyte bacteria were assessed, in order to evaluate the effect of alginate on cell functionality. We showed that mouse peritoneal monocytes stimulated with alginate produce NO and TNF-α. In addition, alginate is able also to increase their phagocytic activity and to a lesser extent also to increase the expression of CD80. Even with different degrees, it implies that alginatesper se act directly on immune response, being able to effectively stimulate proinflammatory activity. These findings corroborate the idea that alginates can represent interesting adjuvants to use to increase the efficacy of antigenic stimulation.

Key words

Monocytes Alginates Immuno-stimulation TNF-α 

References

  1. André, F. E., The future of vaccines, immunisation concepts and practice.Vaccine, 19, 2206–2209 (2001).PubMedCrossRefGoogle Scholar
  2. Bowerestock, T. L. and Martin, S., Vaccine delivery to animals.Advanced Drug Delivery Reviews, 38, 167–194 (1999).CrossRefGoogle Scholar
  3. Cho, N. H., Seong, S. Y., Chun, K. H., Kim, Y. H., Kwon, I. C., Ahn, B. Y., and Jeong, S. Y., Novel mucosal immunization with polysaccharide-protein conjugates entrapped in alginate microspheres.J. Controlled Release, 53, 215–225 (1998).CrossRefGoogle Scholar
  4. Ellis, R. W., Technologies for the design, discovery, formulation and administration of vaccines.Vaccine, 19, 2681–2687 (2001).PubMedCrossRefGoogle Scholar
  5. Espevik, T., Otterlei, M., Skjåk-Braek, G., Ryan, L., Wright, S. D., and Sundan, A., The involvement of CD14 in stimulation of cytokine production by uronic acid polymers.Eur. J. Immunol., 23, 255–261 (1993).PubMedCrossRefGoogle Scholar
  6. Gacesa, P., Bacterial alginate biosynthesis-recent progress and future prospects.Microbiology, 144, 1133–1143 (1998).PubMedCrossRefGoogle Scholar
  7. Gullstein Jahr, T., Ryan, L., Sundan, A., Lichenstein, H. S., Skjåk-Braek, G., and Espevik, T., Induction of tumor necrosis factor production from monocytes stimulated with mannuronic acid polymers and involvement of lipopolysaccharide-binding protein, CD14, and bactericidal/permeability-increasing factor.Infect. Immun., 65, 89–94 (1997).Google Scholar
  8. Hess, J., Schaible, U., Raupach, B., and Kaufmann, S. H. E., Exploiting the Immune System: Toward New Vaccines against Intracellular Bacteria.Adv. Immunlo., 75, 1–88, (2000).CrossRefGoogle Scholar
  9. Kuchroo, V. K., Das, M. P., Brown, J. A., Ranger, A. M., Zamvil, S. S., Sobel, R. A., Weiner, H. L., Nabavi, N., and Glimcher, L. H., B7-1 and B7-2 costimulatory molecules activate differentially the Th1/Th2 development pathways: application to autoimmune disease therapy.Cell, 80, 707–718 (1995).PubMedCrossRefGoogle Scholar
  10. Otterlei, M., Ostgaard, K., Skjåk-Braek, G., Smidsrød, O., Soon-Shiong, P., and Espevik, T., Induction of cytokine production from human monocytes stimulated with alginate.J. Immunother., 10, 286–291 (1991).PubMedCrossRefGoogle Scholar
  11. Otterlei, M., Sundan, A., Skjåk-Braek, G., Ryan, L., Smidsrød, O., and Espevik, T., Similar Mechanisms of action of defined polysaccharides and lipopolysaccharides: characterization of binding and tumor necrosis factor alpha induction.Infect. Immun., 61, 1917–1925 (1993).PubMedGoogle Scholar
  12. Sansom, D. M., Manzotti, C. N., and Zheng, Y., What’s the difference between CD80 and CD86?Trends Immunol., 24, 313–318 (2003).CrossRefGoogle Scholar
  13. Smidsrød, O. and Draget, K. I., Chemistry and physical properties of alginates. Carbohydrates in Europe 1996, 14, 6–12 (1998).Google Scholar
  14. Son, E. H., Moon, E. Y., Rhee, D. K., and Pyo, S., Stimulation of various functions in murine peritoneal macrophages by high mannuronic acid-containing alginate (HMA) exposurein vivo.Int. Immunopharmacol., 1, 147–154 (2001).PubMedCrossRefGoogle Scholar
  15. Tauxe, R. V., Strategies for surveillance and prevention.Lancet, 352 Suppl 4, 10 (1998).CrossRefGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2005

Authors and Affiliations

  • Paolo Pasquali
    • 1
  • Amy Zalcman
    • 1
  • Susanna Murtas
    • 1
  • Rosanna Adone
    • 1
  • Gianfranco Brambilla
    • 1
  • Cinzia Marianelli
    • 2
  • Monica Cagiola
    • 2
  • Franco Ciuchini
    • 1
  1. 1.Dipartimento di Sanità Alimentare ed Animale, Istituto Superiore di SanitàIstituto Superiore di SanitàRomeItaly
  2. 2.Istituto Zooprofilattico dell’Umbria e delle MarchePerugiaItaly

Personalised recommendations