DNA Molecular profiling: a new approach to quality control of chinese drugs

  • Hui Cao
  • Yuping Liu
  • Komatsu Katsuko 
  • But Paul Pui-Hay 
  • Shaw Pang-Chui 
Research progress


DNA technology based molecular profiling provides the ultimate and definitive means of authentication for Chinese drugs. RFLP, RP-PCR and microsatellite-SSR examine the whole genome. With the appropriate primer, it is in principle possible to differentiate closely related species and even one individual from another. PCR-SR, PCR-RFLP, AFLP, SCAR, DALP and DNA sequencing examine a defined region on the genome and produce well-defined conclusions. Since different regions on the genome evolve to different extents, by choosing the target DNA carefully, organisms of different systematic levels can be compared. Of these molecular techniques mentioned above, genomic fingerprints and DNA sequences are equally successful in identification of Chinese drugs. Although RFLP, RP-PCR methods do not require prior to genetic background of the genome and can detect DNA polymorphism, the reliability of these fingerprint results can be greatly influenced by experimental conditions. DNA sequencing method focuses on a defined locus on the genome and may provide a definitive means for authentication of Chinese drugs. We, therefore, conclude that it is essential to combine genomic fingerprinting with DNA sequencing analysis in order to increase the accuracy of quality control.


Internal Transcribe Spacer Restriction Fragment Length Polymorphism Amplify Fragment Length Polymorphism Panax Ginseng American Ginseng 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© The Chinese Journal of Integrated Traditional and Western Medicine Press 2000

Authors and Affiliations

  • Hui Cao
    • 1
    • 4
  • Yuping Liu
    • 1
    • 4
  • Komatsu Katsuko 
    • 2
  • But Paul Pui-Hay 
    • 3
  • Shaw Pang-Chui 
    • 3
  1. 1.Institute of Chinese Materia MedicaChina Academy of TCMBeijing
  2. 2.Toyama Medical and Pharmaceutical UniversityJapan
  3. 3.The Chinese University of Hong KongHKSAR
  4. 4.Now are in the Toyama Medical and Pharmaceutical UniveristyJapan

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