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The effect of acteoside on histamine release and arachidonic acid release in RBL-2H3 mast cells

  • Jin Hee Lee
  • Ji Yun Lee
  • Hyo Suk Kang
  • Chan Hun Jeong
  • Hee Moon
  • Wan Kyunn Whang
  • Chang Jong Kim
  • Sang Soo Sim
Articles Drug Development

Abstract

The effect of acteoside, a phenylpropanoid glycoside isolated fromClerodendron trichotomum Thunberg, on histamine and arachidonic acid release was investigated in RBL 2H3 cells. Histamine was dose-dependently released from RBL 2H3 cells by melittin, arachidonic acid and thapsigargin. In extracellular Ca2+-free solution, basal secretion of histamine increased by two fold. The response of histamine release to melittin and thapsigargin in Ca2+-free solution was significantly decreased, whereas the response to arachidonic acid was significantly increased as compared with those in normal solution. Acteoside inhibited histamine release induced by melittin, arachidonic acid and thapsigargin in a dose-dependent manner in the presence or absence of extracellular Ca2+. However, the inhibitory activity of acteoside was more potent in normal solution than that in Ca2+-free solution. These data suggest that inhibitory mechanism of acteoside on histamine release may be related to extracellular Ca2+. On the other hand, acteoside significantly inhibited arachidonic acid release and prostaglandin E2 production induced by 0.5 μM melittin. It is possible that acteoside may be developed as an anti-inflammatory agent.

Key words

Acteoside Histamine Arachidonic acid Prostaglandin E2 

References

  1. Attur, M. G., Patel, R., Thakker, G., Vyas, P., Levartovsky, D., Patel, P., Naqvi, S., Raza, R., Patel, K., Abramson, D., Bruno, G., Abramson, S. B., and Amin, A. R., Differential anti-inflammatory effects of immunosuppressive drugs: cyclosporin, rapamycin and FK-506 on inducible nitric oxide synthase, nitric oxide, cyclooxygenase-2 and PGE2 production.Inflamm. Res., 49, 20–26 (2000).PubMedCrossRefGoogle Scholar
  2. Battistella, A., Boarato, E., Bruni, A., Mietto, L., Palatini, P., and Toffano, G., Apomorphine-induced inhibition of histamine release in rat peritoneal mast cells.Br. J. Pharmacol., 88, 457–462 (1986).PubMedGoogle Scholar
  3. Choi, J. H., Whang, W. K., and Kim, H. J., Studies on the anti-inflammatory effects of Clerodendron trichotomum Thunberg leaves.Arch. Pharm. Res., 27, 189–193 (2004).PubMedCrossRefGoogle Scholar
  4. Da Silva, A., Amrani, Y., Trifilieff, A., and Landry, Y., Involvement of B2 receptors in the bradykinin-induced relaxation of guinea-pig isolated trachea.Br. J. Pharmacol., 114, 103–108 (1995).PubMedGoogle Scholar
  5. Diaz, A. M., Abad, M. J., Fernandez, L., Silvan, A. M., De Santos, J., and Bermejo, P., Phenylpropanoid glycosides from Scrophularia scorodonia:in vitro anti-inflammatory activity.Life Sci., 74, 2515–2526 (2004).PubMedCrossRefGoogle Scholar
  6. Gueck, T., Seidel, A., and Fuhrmann, H., Consequences of eicosapentaenoic acid (n-3) and arachidonic acid (n-6) supplementation on mast cell mediators.J. Anim. Physiol. Anim. Nutr. (Berl.), 88, 259–265 (2004).CrossRefGoogle Scholar
  7. Jesus, B., Suzanne, E. B., Ismael, D. B., and Edward, A. D., Arachidonic acid mobilization in P388D1 macrophages is controlled by two distinct Ca2+-dependent phospholipase A2 enzymes.Proc. Natl. Acad. Sci. U.S.A., 91, 11060–11064 (1994).CrossRefGoogle Scholar
  8. Kitsukawa, Y., Felley, C., Metz, D. C., and Jensen, R. T., Thapsigargin defines roles of Ca2+ in initial, sustained, and potentiated stimulation of pepsinogen secretion.Am. J. Physiol., 266, 613–623 (1994).Google Scholar
  9. Lau, C. W., Chen, Z. Y., Wong, C. M., Yao, X., He, Z., Xu, H., and Huang, Y., Attenuated endothelium-mediated relaxation by acteoside in rat aorta: Role of endothelial [Ca2+]i and nitric oxide/cyclic GMP pathway,Life Sci., 75, 1149–1157 (2004).PubMedCrossRefGoogle Scholar
  10. Morita, Y., Aida, N., and Miyamoto, T., Role of phospholipase A2 activation in histamine release from human basophils.Allergy, 38, 413–418 (1983).PubMedGoogle Scholar
  11. Nakatani, N., Uozumi, N., Kume, K., Murakami, M., Kudo, I., and Shimizu, T., Role of cytosolic phospholipase A2 in the production of lipid mediators and histamine release in mouse bone-marrow-derived mast cells.Biochem J., 352, 311–317 (2000).PubMedCrossRefGoogle Scholar
  12. Petrone, W. F., English, D. K., Wong, K., and McCord, J. M., Free radicals and inflammation: superoxide-dependent activation of a neutrophil chemotactic factor in plasma.Proc. Natl. Acad. Sci. U.S.A., 77, 1159–1163 (1980).PubMedCrossRefGoogle Scholar
  13. Radvanyi, F., Jordan, L., Russo-Marie, F., and Bon, C., A sensitive and continuous fluorometric assay for phospholipase A2 using pyrene-labeled phospholipids in the presence of serum albumin.Anal. Biochem., 177, 103–109 (1989).PubMedCrossRefGoogle Scholar
  14. Sahpaz, S., Garbacki, N., Tits, M., and Bailleul, F., Isolation and pharmacological activity of phenylpropanoid esters fromMarrubium vulgare.J. Ethnophamacol., 79, 389–392 (2002).CrossRefGoogle Scholar
  15. Shore, P. A., Burkhalter, A., and Cohn, V. H., A method for the fluorometric assay of histamine in tissues.J. Phamacol. Exp. Ther., 127, 182–186 (1959).Google Scholar
  16. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C., Measurement of protein using bicinchoninic acid.Anal. Biochem., 150, 76–85 (1985).PubMedCrossRefGoogle Scholar
  17. Woerdenbag, H. J., Merfort, I., Passreiter, C. M., Schmidt, T. J., Willuhn, G., van Uden, W., Pras, N., Kampinga, H. H., and Konings, A. W., Cytotoxicity of flavonoids and sesquiterpene lactones from Arnica species against the GLC4 and the COLO 320 cell lines.Planta Med., 60, 434–437 (1994).PubMedCrossRefGoogle Scholar
  18. Wong, I. Y., Huang, Y., He, Z. D., Lau, C. W., and Chen, Z. Y., Relaxing effects of Ligstrum purpurascens extract and purified acteoside in rat aortic rings.Planta Med., 67, 317–321 (2001).PubMedCrossRefGoogle Scholar
  19. Xiong, Q., Tezuka, Y., Kaneko, T., Li, H., Tran, L. Q., Hase, K., Namba, T., and Kadota, S., Inhibition of nitric oxide by phenylethanoids in activated macrophages.Eur. J. Pharmacol., 14, 137–144 (2000).CrossRefGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2006

Authors and Affiliations

  • Jin Hee Lee
    • 1
  • Ji Yun Lee
    • 1
  • Hyo Suk Kang
    • 1
  • Chan Hun Jeong
    • 1
  • Hee Moon
    • 1
  • Wan Kyunn Whang
    • 1
  • Chang Jong Kim
    • 1
  • Sang Soo Sim
    • 1
  1. 1.Department of Phathophysiology, College of PharmacyChung-Ang UniversitySeoulKorea

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