Archives of Pharmacal Research

, Volume 29, Issue 7, pp 577–581 | Cite as

Glycation-induced inactivation of antioxidant enzymes and modulation of cellular redox status in lens cells

  • Ai Hyang Shin
  • Chang Joo Oh
  • Jeen-Woo Park
Articles Drug Devopment


Oxidative mechanisms are thought to have a major role in cataract formation and diabetic complications. Antioxidant enzymes play an essential role in the antioxidant system of the cells that work to maintain low steady-state concentrations of the reactive oxygen species. When HLE-B3 cells, a human lens cell line were exposed to 50–100 mM glucose for 3 days, decrease of viability, inactivation of antioxidant enzymes, and modulation of cellular redox status were observed. Significant increase of cellular oxidative damage reflected by lipid peroxidation and DNA damage were also found. The glycation-mediated inactivation of antioxidant enzymes may result in the perturbation of cellular antioxidant defense mechanisms and subsequently lead to a pro-oxidant condition and may contribute to various pathologies associated with the long term complications of diabetes.

Key words

Glycation Diabetes Antioxidant enzymes Redox status Lens cells 


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Copyright information

© The Pharmaceutical Society of Korea 2006

Authors and Affiliations

  1. 1.School of Life Sciences and Biotechnology, College of Natural SciencesKyungpook National UniversityTaeguKorea

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