Glycation-induced inactivation of antioxidant enzymes and modulation of cellular redox status in lens cells
Oxidative mechanisms are thought to have a major role in cataract formation and diabetic complications. Antioxidant enzymes play an essential role in the antioxidant system of the cells that work to maintain low steady-state concentrations of the reactive oxygen species. When HLE-B3 cells, a human lens cell line were exposed to 50–100 mM glucose for 3 days, decrease of viability, inactivation of antioxidant enzymes, and modulation of cellular redox status were observed. Significant increase of cellular oxidative damage reflected by lipid peroxidation and DNA damage were also found. The glycation-mediated inactivation of antioxidant enzymes may result in the perturbation of cellular antioxidant defense mechanisms and subsequently lead to a pro-oxidant condition and may contribute to various pathologies associated with the long term complications of diabetes.
Key wordsGlycation Diabetes Antioxidant enzymes Redox status Lens cells
Unable to display preview. Download preview PDF.
- McCord, J. M. and Fridovich, I., Superoxide dismutase: An enzymic function for erythrocuprein (hemocuprein).J. Biol. Chem., 224, 6049–6055 (1969).Google Scholar
- Nishikawa, T., Edelstein, D., Du, X. L., Yamagishi, S., Matsumura, T., Kaneda, Y., Yorek, M. A., Beebe, D., Oates, P. J., Hammes, H. P., Giardino, I., and Brownlee, M., Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage.Nature, 404, 787–790 (2001).Google Scholar
- Varma, S. D. and Kinoshita, J. H., Sorbitol pathway in diabetic and galactosemic rat lens.Biochim. Biophys. Acta, 328, 632–640 (1974).Google Scholar