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Chinese Journal of Cancer Research

, Volume 10, Issue 4, pp 239–242 | Cite as

The role of recombinant Rb gene adenovirus vector in the growth of lung adenocarcinoma cells

  • Li Jian 
  • Jiang Lei 
  • Xia Yongjing 
  • Li Hongxia 
  • Hu Yajun 
  • Hu Shixue 
  • Xu Hongji 
Article
  • 17 Downloads

Abstract

Objective: To study the role of the most extensively studied tumor suppressor gene, retinoblastoma (Rb) gene, on the growth of lung adenocarcinoma cell line GLC-82 and explore a gene therapy approach for lung adenocarcinoma. Methods: The recombinant Rb gene adenovirus vector was constructed, the control virus which carries LacZ gene was producted by the same method. Infection effects were detected by biochemical staining of β-gal and immunohistochemical analysis of Rb protein. The Rb cDNA of infected cells were determined by PCR. The cell growth rate and cell cycle were observed by cell-counting and flow cytometry. Results: The constructed recombinant adenovirus vector could infect effectively the cells with high level expression of Rb cDNA and Rb protein. The transfection of wild-type Rb gene could suppress GLC-82 cell proliferation and decrease the cellular DNA synthesis. Conclusions: These results showed the possibility of using recombinant Rb gene adenovirus vector in the gene therapy of cancer to inhibit the growth of cancer.

Key words

Adenovirus vector Retinoblastoma gene GLC-82 cell 

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Copyright information

© Chinese Journal Of Cancer Research 1998

Authors and Affiliations

  • Li Jian 
    • 1
  • Jiang Lei 
    • 1
  • Xia Yongjing 
    • 1
  • Li Hongxia 
    • 1
  • Hu Yajun 
    • 1
  • Hu Shixue 
    • 2
  • Xu Hongji 
    • 2
  1. 1.Beijing Institute of GeriatricsBeijing HospitalBeijingChina
  2. 2.Department of Molecular OncologyThe University of Texas MD Anderson Cancer CenterHoustonUSA

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