Abstract
Objective: To study the role of the most extensively studied tumor suppressor gene, retinoblastoma (Rb) gene, on the growth of lung adenocarcinoma cell line GLC-82 and explore a gene therapy approach for lung adenocarcinoma. Methods: The recombinant Rb gene adenovirus vector was constructed, the control virus which carries LacZ gene was producted by the same method. Infection effects were detected by biochemical staining of β-gal and immunohistochemical analysis of Rb protein. The Rb cDNA of infected cells were determined by PCR. The cell growth rate and cell cycle were observed by cell-counting and flow cytometry. Results: The constructed recombinant adenovirus vector could infect effectively the cells with high level expression of Rb cDNA and Rb protein. The transfection of wild-type Rb gene could suppress GLC-82 cell proliferation and decrease the cellular DNA synthesis. Conclusions: These results showed the possibility of using recombinant Rb gene adenovirus vector in the gene therapy of cancer to inhibit the growth of cancer.
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This study was supported in part by grant from National Nature Science Foundation of China (No. 39570775)
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Li, J., Jiang, L., Xia, Y. et al. The role of recombinant Rb gene adenovirus vector in the growth of lung adenocarcinoma cells. Chinese Journal of Cancer Research 10, 239–242 (1998). https://doi.org/10.1007/BF02962169
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DOI: https://doi.org/10.1007/BF02962169