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A method for the direct estimation of total serum thyroxine

  • P. P. A. Smyth
  • D. K. O’Donovan
Article

Summary

Serum is deprotenized using 95% ethanol. The T4 thus released from the protein binding is quantitated by its competition with a fixed amount of T4123I for binding sites on a fixed amount of TBG. The mean value for thyroxine iodine in a group of 72 clinically euthyroid subjects is 5.94±1.34 g/100mls. giving a normal range of 3.3–8.6 μg/100mls. Agreement with the elinical status in a group of 130 subjects under assessment is 96.2%.

As well as providing a direct measurement of circulating thyroxine the method is not affected by the contaminants which invalidate the P.B.I. estimation.

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Copyright information

© Springer 1968

Authors and Affiliations

  • P. P. A. Smyth
    • 1
  • D. K. O’Donovan
    • 1
  1. 1.Department of Medicine and TherapeuticsUniversity CollegeDublin

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