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Sugar Tech

, Volume 9, Issue 1, pp 36–42 | Cite as

Effects of molybdenum on nitrogen metabolism of sugarcane

  • Li -Ping Wei
  • Yang -Rui Li
  • Li -Tao Yang
Research Article

Abstract

Two nitrogen fixing sugarcane varieties of Brazil RB72/454 and RB86/7575 were employed as the plant materials. They were cultured in nutrient solution containing different levels of Mo i.e. 0mg/L, 0.01mg/L, 0.02mg/L and 0.04mg/L, respectively, in pot trial with sand in greenhouse. The activities of nitrate reductase (NR) and glutamine synthetase (GS), and the contents of NO3 -N and NH4 +-N were measured at different stages. The results showed that under nitrogenous conditions in the greenhouse, all the Mo treatments increased the activity of NR and decreased the NO3 -N content compared with the control for both the test varieties. As a result, the treatment with 0.01mg/L Mo increased the activity of GS in RB72/454 after initial elongation stages and that in RB86/7575 at young seedling stage, and it also decreased the NH4 -N content to improve the nitrogen metabolism. Under non-nitrogenous conditions, after initial elongation stage, the activity of NR was increased and the NO3 -N content was decreased in both sugarcane varieties treated with different levels of Mo. Furthermore, the activity of GS was higher than the control of RB72/454 and the NH4 +-N content was lower in leaves of RB86/7575 at tillering stage. The effect of different Mo treatment on the process of nitrogen metabolism and ammonia assimilation at different stages of growth was different under nitrogenous or non-nitrogenous condition. The lower concentration of Mo could increase the activities of NR and GS absolutely, decrease the contents of NO3 -N and NH4 +-N in leaf of the two sugarcane varieties as well as improve the nitrogen metabolism and ammonia assimilation.

Key words

sugarcane molybdenum nitrogen metabolism 

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References

  1. Arnon, D.I. (1958). The role of microelements in plant nutrition with special reference to photosynthesis and nitrogen assimilation[A]. In: Lamb C A, Barow N, Sei D J(eds). Trace elements. New York: Elserier Scientific Publishering Company, pp.123–136.Google Scholar
  2. Bortels, H. (1930). Molybdenum as catalyzer in biological nitrogen- fixation.Arch Mikrobior,1: 333–342.CrossRefGoogle Scholar
  3. Boquinhok, X.H. (1981). Methods of Plant Biochemical Analysis. Science Press, Beijing, pp.95–100.Google Scholar
  4. Chao, Z.Q. (1997). Study on molybdenum nutrition of peanut. Peanut Science and Technology, (03):23–26.Google Scholar
  5. Du, Y.Q., Wang, Y.H., Wei, W.X. andWang, Z.R. (1994). Effect of molybdenum fertilizer application on the nitrogen and molybdenum nutritions of wheat.Journal of Huazhoung Agricultural University,13(04):384–389.Google Scholar
  6. Du, Y.Q., Liao, X.R., Huang, Z.Y., He, J.H., Zhou, X.H. andYuan C.T. (2001). Effect of boron and molybdenum on nitrogen metabolism of peanut.Acta Agronomica Sinica,27(5):612–616.Google Scholar
  7. Du, Y.Q., Liao, X.R., He, J.H., Huang, Z.Y. andZhou, X.H. (2002). Effects of boron and molybdenum on growth and yield of peanut.Plant Natrition and Fertilizen Science,8(2):229–233.Google Scholar
  8. Lam, H.M., Coschigano, K.T., Oliveira, I.C., Melo-Oliveira, R. andCoruzzi, G.M. (1996). The molecular genetics of nitrogen assimilation into amino acids in higher plants.Annu. Rev. Plant Physiol. Plant Mol. Biol.,47: 569–593.PubMedCrossRefGoogle Scholar
  9. Liu, P. andYang, Y.A. (1999). Effect of molybdenum and boron on nitrogen metabolism of soybean.Plant Natrition and Fertilizen Science,5(4):347–351.Google Scholar
  10. Liu, P. andYang, Y.A. (2000). The effect of the stress of boron and molybdenum on NR activity and contents of nitrate-N in leaves of soybean.Journal of Zhejiang Agricultural University (Agric.& Life Sci.),26(2): 151–154.Google Scholar
  11. Li, H.S. (2000). Experimental Mechanism and Technology of Plant Physiology and Biochemisty. Higher Grade Education Press, Beijing. pp.123–124.Google Scholar
  12. Plant Physiological Teaching and Research Section, Biology Department of East China Normal University (eds). (1980). Experimental Guide of Plant Physiology. Shanghai Science Press, Shanghai, pp.73–75.Google Scholar
  13. Sun, X.C., Hu, C.X., Tan, Q.L., Wei, W.X. andWang, Y.H. (2002). Effects of molybdenum application on contents of free amino acid, soluble sugar and protein of winter wheat at different growth stages.Journal of Huazhoung Agricultural University,21(1):40–43.Google Scholar
  14. Wu, M.C. andXiao, C.Z. (1994). Study on molybdenum soybean.Soybean Sci.,13(03):245–251.Google Scholar
  15. Wu, J.G. andLou, D.R. (1996). Effects of applying molybdenum on crops and complementary effects of molybdenum and phosphorus. Jiangsu Agricultural Sciences, (02):40–42.Google Scholar
  16. Wei, W.X., Tan, Q.L. andWang, Y.H. (1996). Studies of the effect of molybdenum nutrition on the balance of nutrients of winter wheat.Journal of Huazhoung Agricultural University,15(05):437–441.Google Scholar
  17. Zhou, S. andZheng, X.M. (1985). Analysis method on nitrate reductase in plant.Plant Physiology Communications, (1):47–49.Google Scholar
  18. Zou, Q. (1999). Experimental Guide of Plant Physiology. Science Press, Beijing, pp.56–125.Google Scholar

Copyright information

© Society for Sugar Research & Promotion 2007

Authors and Affiliations

  • Li -Ping Wei
    • 1
    • 2
  • Yang -Rui Li
    • 1
    • 2
    • 3
  • Li -Tao Yang
    • 1
  1. 1.Guangxi Academy of Agricultural SciencesNanningChina
  2. 2.Sugarcane Research CenterChinese Academy of Agricultural SciencesNanningChina
  3. 3.Agriculture CollegeGuangxi UniversityNanningChina

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