Abstract
The meristem tip culture in combination with antiviral chemotherapy was found significant to enhance the sugarcane mosaic virus (SCMV) elimination from the infected sugarcane meristem. Two antiviral chemotherapeutants, viz. ribavirin and 8-azaguanine were applied with MS medium in different concentrations (5, 10, 25 and 50 ppm) and meristem culture was done. The SCMV elimination was assessed in direct antigen coating (DAC)-ELISA with polyclonal antiserum raised against SCMV. Amending the antiviral chemotherapeutant, ribavirin at 50 ppm with MS medium was found promising in SCMV elimination up to 95 per cent when compared to 62 per cent in meristem culture alone and ribavirin did not exhibit any phytotoxicity on sugarcane. However, the other chemotherapeutant, 8-azaguanine exhibited phytotoxicity and reduced regeneration of plantlets. The results indicated that the combined method of antiviral chemotherapy with ribavirin (50 ppm) and meristem culture was effective in SCMV elimination when compared to meristem culture alone.
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References
Agnihotri, V.P. (1996). Current sugarcane disease scenario and management strategies.Indian Phytopath.,49: 10–126.
Balamuralikrishnan, M. (2001). Molecular characterization, detection, economic impact, resistance mechanism and elimination of sugarcane mosaic potyvirus in sugarcane. PhD thesis, Tamil Nadu Agricultural University, Coimbatore, India.
Cassells, A.C. and Long, R.D. (1982). The elimination of potato viruses X, Y, S and M in meristem and potato virus Y infected tobacco explants cultured in the presence of virazole. Z.Naturforsch. Teil. C.,35: 356–359.
Costa, A.S. and Muller, G.W. (1982). General evaluation of the impacts of virus diseases of economic crops on the development of Latin American Countries. In: Proc. Conf. Impact Virus Diseases Dev. Latin American Caribbean Countries, Rio De, Janeiro.
Dawson, O. and Lozoya-Saldana, H. (1984). Examination of the mode of action of ribavirin against tobacco mosaic virus.Intervirology,22: 77–80.
El-Nasr, A.M.A., Fahmy, F.G. and Fushdi, M.H. (1989). Elimination of sugarcane mosaic disease by tissue culture and hot water treatment.Asian J. Agric. Sci.,20: 277–292.
Gopal, K. and Reddy, K.S. (1988). Studies on mosaic disease of sugarcane (Saccharum officinarum L.).Plant Pathology Newsletter, 6:1–2.
Goswami, B.B., Crea, R., Van Boom, J.H. and Sharma, O.H. (1982). An approach to inhibition of viral replication: Inhibition of mRNA methylation.Cancer Res. Clin. Oncol.,103: 321.
Hansen, A.J. (1985). An end to the dilemma virus free all the way.Hort. Science,20: 852–858.
Hansen, A.J. (1989). Antiviral chemicals for plant disease control.Crit. Rev. Plant Sci.,8(1): 45–81
Hansen, A.J. and Lane, W.D. (1985). Elimination of apple chlorotic leafspot virus from apple shoot cultures by ribavirin.Plant Dis.,69: 134–137.
Hendre, R.R., Mascarenhas, A.F., Nadgri, A.L., Pathak, M. and Jagannathan, V. (1975). Growth of mosaic virus free sugarcane plants from apical meristems.Indian Phytopath.,28: 175–178.
Irvine, J.E. and Benda, G.T.A. (1985). Sugarcane mosaic virus in plantlets regenerated from diseased leaf tissue.Plant Cell Tissue Organ Culture,5(1): 101–106.
Joshi, R.D. and Prakash, J. (1978). Inhibition of sugarcane mosaic virus by plant rhizome extracts.Int. Sugar J.,80(954): 173–174.
Lerch. B. (1987). On the inhibition of plant virus multiplication by ribavirin.Antiviral Res.,7: 257,
Molina, M. and Leon, O. (1991). Antiviral activity of leaf extracts ofBougainvillea spectabilis, Capsicum annuum, Datura metal and Datura stramonium on sugarcane mosaic virus.Revista de Proteccion Vegetal.,6: 150–155.
Molina, M. and Sanchez, L.M. (1993). Effectivity and chemical nature of leaf extractsDatura stramonium as inhibitors of sugarcane mosaic virus.Revista de Protection Vegetal.,8: 267–272.
Mowat, W.P. and Dawson, S. (1987). Detection and identification of plant viruses by ELISA using crude sap extracts and unfractionated antisera.J. Virol. Meth.,15: 233–247.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures.Physiologia Plant,15: 473–497.
Rott, P., Bailey, R.A., Comstock, J.C., Craft, B.J. and Saumtally, A.S. (2000). A Guide to Sugarcane Diseases, CIRAD-ISSCT, CIRAD Publication services, Montepellier, France, p. 339.
Shukla, K. (1978). Inhibitory effect of some heteropolyamines on sugarcane mosaic virus.Int. Sugar J.,80: 263–264.
Shukla, K. and Joshi, R.D. (1979). Influence of some phenolic acids on the infectivity of sugarcane mosaic virus.Sugarcane Pathologists Newsl.,23: 19–21.
Singh, S.P., Rao, G.P., Singh, J. and Singh, S.B. (1997). Effect of sugarcane mosaic poty virus infection on metabolic activity, yield and juice quality.Sugar Cane,5: 19–23.
Smith, G.R., Ford, F. Frenkel, M.J. Shukla, D.D. and Dale.J.L. (1992). Transient expression of the coat protein of sugarcane mosaic virus in sugarcane protoplasts and expression inEscherichia coli. Arch Virol.,125: 19–23.
Stace-Smith, R. (2000). Tissue culture. In: Mandahar, eds. Plant Viruses Vol. III: CRC Press, Boston, USA, pp. 295–300.
Steib, R.J. and Cifuentes, O.M. (1977). Use of aerated steam as a possible method for the control of sugarcane mosaic and ratoon stunting disease.Sugarcane Pathologists Newsl.,18: 24–27.
Xu, L.P., Chen, R.K. and Li, Y.P. (1994). Elimination of sugarcane mosaic virus by callus tissue culture and apical culture.J. Fujian Agric. Univ.,23: 253–256.
Viswanathan, R. and Mohanraj, D.(2001). Detection of sugarcane viral diseases by serological, techniques. In: Sugarcane pathology. Vol. II. Virus and phytoplasma diseases (Eds. G.P. Rao, R.E. Ford, M. Tosic and D.S. Teakle). Oxford & IBH Publishers Co. Pvt. Ltd., New Delhi, pp 195–208.
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Balamuralikrishnan, M., Doraisamy, S., Ganapathy, T. et al. Combined effect of chemotherapy and meristem culture on sugarcane mosaic virus elimination in sugarcane. Sugar Tech 4, 19–25 (2002). https://doi.org/10.1007/BF02956875
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DOI: https://doi.org/10.1007/BF02956875